Hum. Reprod. Advance Access published online on March 3, 2005
Human Reproduction, doi:10.1093/humrep/deh758
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1 3121 S. Maryland Pkwy, Ste 300, Las Vegas, NV 89109, USA; Sher Institutes for Reproductive Medicine, Las Vegas, NV 89109, USA
* To whom correspondence should be addressed. BACKGROUND: We have previously reported the retrospective observation that when at least one embryo, transferred on day 3, expressed sHLA-G above the geometric mean (sHLA-G+) 46 h post-ICSI, there was a marked improvement in both pregnancy (PR) and implantation (IR) rates. METHODS: The media surrounding individual embryos derived from ICSI performed on oocytes from 482 women
Received October 8, 2004
Revised November 22, 2004
Accepted December 21, 2004
Article
Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study
2 Sher Institutes for Reproductive Medicine, Las Vegas, NV 89109, USA
3 Mobilab, Las Vegas, NV 89109, USA
G. Sher, E-mail: gsher{at}sherinstitute.com
Abstract 
43 years of age were tested for sHLA-G expression by specific ELISA. RESULTS: We report here prospective results showing improved IVF results following the transfer of ‘good quality’ embryos (7-9 cells with <20% fragmentation) by preferentially including at least one sHLA-G+ embryos. PR and IR for women 38 years were 63% and 32% when one transferred embryo was sHLA-G+, and 69% and 36% when at least two embryos were sHLA-G+. When none of the embryos transferred was sHLA-G+, PR and IR were 25% and 13%, respectively. Comparable PR and IR for women 39-43 years were 29% and 11% when none of the transferred embryos were sHLA-G+; 38% and 15% when at least one sHLA-G+ embryo was transferred; and 61% and 26% when at least two 2 sHLA-G+ embryos were transferred. The data were stratified by patient age. CONCLUSIONS: PR and IR increased with the addition of each sHLA-G+ embryo, regardless of age. While there are significant barriers to routine embryo sHLA-G testing, we believe that if implemented, this would provide a mechanism for optimizing IVF PR while minimizing the risk of multiple pregnancies.
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