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Hum. Reprod. Advance Access published online on February 25, 2005
Human Reproduction, doi:10.1093/humrep/deh780
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Human Reproduction © European Society of Human Reproduction and Embryology 2005; all rights reserved
Received November 10, 2004
Revised December 17, 2004
Accepted January 11, 2005

Article

The use of enzyme-linked immunosorbent assay for detection of Mycoplasma hominis antibodies in infertile women serum samples

Agata Baczynska 1*, Helle Friis Svenstrup 2, Jens Fedder 3, Svend Birkelund 2, and Gunna Christiansen 2

1 Department of Medical Microbiology and Immunology, Aarhus University, The Bartholin Building, 8000 Aarhus C, Denmark and The Fertility Clinic & The Scientific Unit, Brædstrup and Horsens Hospitals, 8740 Brædstrup, Denmark
2 Department of Medical Microbiology and Immunology, Aarhus University, The Bartholin Building, 8000 Aarhus C, Denmark
3 The Fertility Clinic & The Scientific Unit, Brædstrup and Horsens Hospitals, 8740 Brædstrup, Denmark

* To whom correspondence should be addressed.
Agata Baczynska, E-mail: agata{at}medmicro.au.dk


  Abstract

BACKGROUND: Besides Chlamydiae trachomatis and Mycoplasma genitalium, Mycoplasma hominis may also cause infertility due to damage of the Fallopian tubes. Therefore serum samples from infertile women were analyzed for antibodies to M. hominis. METHODS: Sera from 304 infertile women were investigated for seropositivity to M. hominis by immunoblotting and a developed ELISA. Women were classified into groups based on the type of infertility: infertile due to lack of passage in Fallopian tubes (TFI, tubal factor infertility), an infertile male partner (MFI, male factor infertility) and unexplained infertility (UFI, unexplained factor infertility). Three M. hominis isolates were used in the immunoblotting analysis and clear differences in patient immunoprofiles were observed between two isolates. For the ELISA we used a mixture of Triton X-114 extracted membrane proteins from those two M. hominis isolates as antigen. RESULTS: Ninety-seven sera (32%) were seropositive to M. hominis when tested by the ELISA. There was a significant correlation between TFI and seropositivity to M. hominis (P=0.0015, OR = 2.21, CI = 1.35-3.61). We compared the seropositivity of 304 patients to M. hominis with the presence of antibodies against two other bacteria Chlamydiae trachomatis and Mycoplasma genitalium and there was no statistical correlation between those bacteria and M. hominis. CONCLUSION: Our results indicate that M. hominis may be an independent predictor of TFI.

Keywords: antibodies; ELISA; immunoblot; membrane proteins; Mycoplasma hominis.
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