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Hum. Reprod. Advance Access published online on March 10, 2005
Human Reproduction, doi:10.1093/humrep/deh826
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Human Reproduction © European Society of Human Reproduction and Embryology 2005; all rights reserved
Received December 1, 2004
Revised January 26, 2005
Accepted January 31, 2005

Article

Transfer of germinal vesicle to ooplasm of young mice could not rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice

Long-Bo Cui 1, Xiu-Ying Huang 2, and Fang-Zhen Sun 2*

1 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China and Department of Biology, Yantai University, Yantai 264005, China
2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China

* To whom correspondence should be addressed.
Fang-Zhen Sun, E-mail: fzsun{at}genetics.ac.cn


  Abstract

BACKGROUD: Transferring a germinal vesicle (GV) from an aged woman's oocyte into ooplasm from a younger woman has been proposed as a possible way to overcome the problem of age-related decline in female fertility. Here we assessed this possibility by determining whether ooplasts derived from young mice could rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice. METHODS: Three groups of reconstructed oocytes, young GV-young cytoplast (group YY), aged GV-young cytoplast (group AY), and young GV-aged cytoplast (group YA), were created by micromanipulation and electrofusion. RESULTS: Nuclear transplantation was successful in 89.8-94.4% of GV-ooplast complexes, and maturation rate of the reconstructed oocytes was 93.5-97.9%. Confocal microscopy analysis showed a significantly higher rate (49.2%) of chromosome misalignment in ageing mice than in young mice (16.9%), and 57.1% of oocytes in group AY exhibited chromosome misalignment, while the abnormality rate in groups YY and YA was 16.3 and 16.7% respectively. Calcium imaging showed that the three groups of reconstructed oocytes exhibited a similar pattern of calcium oscillations upon stimulation with bovine sperm extracts. Fertilization rate and developmental capacity to 2-cell embryos were also similar among the three groups of oocytes. CONCLUSIONS: Our findings suggest that: (i) the ooplasm from young mice could not rescue ageing-associated chromosome misalignment in meiosis of GV from aged mice; and (ii) behaviour of chromosome alignment over metaphase spindle is predominantly determined by GV material.

Keywords: ageing; Ca2+ oscillations; Chromosome; germinal vesicle transfer; oocyte.
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