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Hum. Reprod. Advance Access published online on March 31, 2005
Human Reproduction, doi:10.1093/humrep/deh868
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received March 15, 2004
Revised February 22, 2005
Accepted February 24, 2005

Article

Meiotic anomalies in infertile men with severe spermatogenic defects

M.R. Guichaoua 1*, J. Perrin 1, C. Metzler-Guillemain 2, J. Saias-Magnan 2, R. Giorgi 3, and J.M. Grillo 1

1 Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale (EA1784), IFR PMSE112, Faculté de Médecine Timone, 27, Boulevard Jean Moulin, 13385, Marseille cedex 05, France; Laboratoire de Biologie de la Reproduction, Hôpital de la Conception, 147, Bd Baille, 13385 Marseille cedex 5, France
2 Laboratoire de Biologie de la Reproduction, Hôpital de la Conception, 147, Bd Baille, 13385 Marseille cedex 5, France
3 Laboratoire d'enseignement et de recherche sur le traitement de l'Information Médicale (LERTIM), Faculté de Médecine, 27, Boulevard Jean Moulin, 13385 Marseille cedex 05, France

* To whom correspondence should be addressed.
M.R. Guichaoua, E-mail: mguichaoua{at}ap-hm.fr


  Abstract

BACKGROUND: This study was aimed at evaluating the rate of pairing failure in pachytene spermatocytes of patients presenting either an obstructive (O) or a non-obstructive (NO) infertility. METHODS: Forty-one patients and 13 controls underwent testicular biopsy. Among the patients, 19 had an O infertility and 22 a NO infertility. Preparations of all patients and controls were Giemsa-stained, and synaptonemal complexes from nine of these patients and one control were immunostained. RESULTS: In all, 2931 pachytene nuclei were analysed. The mean rate of asynapsed nuclei from the NO group (25.4%) was significantly higher than that of the O group (9.8%). There was no significant difference between the O group and the controls (10.6%). Immunocytochemistry showed that the number of pachytene nuclei decreased from the early to late pachytene sub-stage in all patients. Two NO patients, one azoospermic and one oligozoospermic, had a high percentage of asynapsed nuclei (86 and 91.8% respectively); one of these patients also presented a precocious localized separation of sister chromatids. CONCLUSION: high levels of extended asynapsis could arise from a primary meiotic defect which may be responsible for 9% of the NO male infertilities at our centre. The prevalence of early pachytene substages suggests that the pachytene checkpoint is localized at the mid-pachytene stage in humans.

Keywords: asynapsis; meiosis; pachytene checkpoint; pachytene stage; spermatogenic failure.
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