Skip Navigation



Hum. Reprod. Advance Access published online on May 12, 2005
Human Reproduction, doi:10.1093/humrep/dei023
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/8/2133    most recent
dei023v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Codina-Pascual, M.
Right arrow Articles by Benet, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Codina-Pascual, M.
Right arrow Articles by Benet, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

©The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received September 16, 2004
Revised February 14, 2005
Accepted March 14, 2005

Article

Synapsis and meiotic recombination analyses: MLH1 focus in the XY pair as an indicator

Montserrat Codina-Pascual 1*, Maria Oliver-Bonet 1, Joaquima Navarro 1, Mercè Campillo 2, Ferran García 3, Susana Egozcue 3, Carlos Abad 4, Josep Egozcue 1, and Jordi Benet 1*

1 Unitat de Biologia i Genètica Mèdica, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
2 Laboratori de Medicina Computacional, Unitat de Bioestadística, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
3 Unitat Andrologia, Institut Marquès, 08024 Barcelona, Spain and
4 Servei d'Urologia, Consorci Hospitalari Parc Taulí, 08208 Sabadell, Spain

* To whom correspondence should be addressed.
Montserrat Codina-Pascual, E-mail: jordi.benet{at}uab.es, montserrat.codina@uab.es
Jordi Benet, E-mail: jordi.benet{at}uab.es, montserrat.codina@uab.es


  Abstract

BACKGROUND: Anomalies in meiotic prophase I have been related to partial or total meiotic arrest. These anomalies include an abnormal synaptic process, resulting in disorders in meiotic recombination. METHODS: In the present study, we analyse primary spermatocytes from 12 infertile men (four with non-obstructive azoospermia, six with oligoastenoteratozoospermia, one with astenoteratozoospermia and one normozoospermic) and five control fertile donors using immunocytological techniques for synaptonemal complex, meiotic recombination and centromeric proteins. RESULTS: Mean numbers of MLH1 foci per cell, frequencies of cells presenting an MLH1 focus in the XY pair and percentages of cells affected by abnormal synaptic patterns (gaps and splits) are reported for each of the infertile patients and control men. A positive correlation between the frequency of cells showing a recombination focus in the XY pair and the number of autosomal recombination foci per cell is found. CONCLUSIONS: Reduced recombination in the XY pair and an increased number of cells affected by gaps may explain some idiopathic male infertility cases. The results suggest that recombination in the XY pair could be an indicator for general recombination frequency and for a successful meiotic process.

Keywords: male infertility; meiotic recombination; pachytene; synaptonemal complexes; XY pair.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
A. Pinton, A. Calgaro, N. Bonnet, S. Ferchaud, S. Billoux, A.M. Dudez, N. Mary, K. Massip, A. Bonnet-Garnier, M. Yerle, et al.
Influence of sex on the meiotic segregation of a t(13;17) Robertsonian translocation: a case study in the pig
Hum. Reprod., August 1, 2009; 24(8): 2034 - 2043.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Lian, Y. Yin, M. Oliver-Bonet, T. Liehr, E. Ko, P. Turek, F. Sun, and R. H. Martin
Variation in crossover interference levels on individual chromosomes from human males
Hum. Mol. Genet., September 1, 2008; 17(17): 2583 - 2594.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
F. Sun, M. Mikhaail-Philips, M. Oliver-Bonet, E. Ko, A. Rademaker, P. Turek, and R.H. Martin
The relationship between meiotic recombination in human spermatocytes and aneuploidy in sperm
Hum. Reprod., August 1, 2008; 23(8): 1691 - 1697.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
R.H. Martin
Cytogenetic determinants of male fertility
Hum. Reprod. Update, June 4, 2008; (2008) dmn017v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Oliver-Bonet, M. Campillo, P. J. Turek, E. Ko, and R.H. Martin
Analysis of replication protein A (RPA) in human spermatogenesis
Mol. Hum. Reprod., December 1, 2007; 13(12): 837 - 844.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
F. Sun, M. Oliver-Bonet, T. Liehr, H. Starke, P. Turek, E. Ko, A. Rademaker, and R. H. Martin
Variation in MLH1 distribution in recombination maps for individual chromosomes from human males
Hum. Mol. Genet., August 1, 2006; 15(15): 2376 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Codina-Pascual, J. Navarro, J. Egozcue, and J. Benet
A human tetraploid pachytene spermatocyte as the possible origin of diploid sperm: a case report
Hum. Reprod., July 1, 2006; 21(7): 1795 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Codina-Pascual, J. Navarro, M. Oliver-Bonet, J. Kraus, M.R. Speicher, O. Arango, J. Egozcue, and J. Benet
Behaviour of human heterochromatic regions during the synapsis of homologous chromosomes
Hum. Reprod., June 1, 2006; 21(6): 1490 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Ma, K.A. Ferguson, S. Arsovska, P. Moens, and V. Chow
Reduced recombination associated with the production of aneuploid sperm in an infertile man: a case report
Hum. Reprod., April 1, 2006; 21(4): 980 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Codina-Pascual, M. Campillo, J. Kraus, M.R. Speicher, J. Egozcue, J. Navarro, and J. Benet
Crossover frequency and synaptonemal complex length: their variability and effects on human male meiosis
Mol. Hum. Reprod., February 1, 2006; 12(2): 123 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
F. Sun, M. Oliver-Bonet, T. Liehr, H. Starke, K. Trpkov, E. Ko, A. Rademaker, and R. H. Martin
Discontinuities and unsynapsed regions in meiotic chromosomes have a cis effect on meiotic recombination patterns in normal human males
Hum. Mol. Genet., October 15, 2005; 14(20): 3013 - 3018.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.