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Hum. Reprod. Advance Access published online on April 21, 2005
Human Reproduction, doi:10.1093/humrep/dei032
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received September 8, 2004
Revised March 7, 2005
Accepted March 29, 2005

Article

Expression of inhibin {alpha}, glial cell line-derived neurotrophic factor and stem cell factor in Sertoli cell-only syndrome: relation to successful sperm retrieval by microdissection testicular sperm extraction

K. Fujita 1, A. Tsujimura 1*, T. Takao 1, Y. Miyagawa 1, K. Matsumiya 1, M. Koga 2, M. Takeyama 2, H. Fujioka 3, K. Aozasa 4, and A. Okuyama 1

1 Department of Urology and , Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita Osaka 565-0871, Japan
2 Department of Urology, Osaka Central Hospital, Osaka, 530-0001, Japan and
3 Department of Urology, Osaka Police Hospital, Osaka, 543-8502, Japan
4 Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita Osaka 565-0871, Japan,

* To whom correspondence should be addressed.
A. Tsujimura, E-mail: akitsuji{at}uro.med.osaka-u.ac.jp


  Abstract

BACKGROUND: Microdissection testicular sperm extraction (TESE) has provided new hope for successful sperm retrieval to patients with Sertoli cell-only syndrome (SCO). We determined expression of the inhibin {alpha} subunit, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in Sertoli cells obtained from patients with SCO immunohistochemically and compared expression rates with rates of microdissection TESE sperm retrieval. METHODS: Testicular biopsy specimens were obtained from 52 men with non-obstructive azoospermia who underwent microdissection TESE and were diagnosed with SCO by histological analysis. RESULTS: All specimens showed intense staining for the inhibin {alpha} subunit. Moderate or intense staining for GDNF was observed in 65.8% of specimens. All but one showed moderate or intense staining for SCF. Among specimens negative for GDNF, the sperm retrieval rate was significantly higher (100%) for specimens with intense staining for SCF than for specimens with no or moderate staining (30.7%) (P<0.05) for SCF. CONCLUSION: GDNF expression differs among patients with SCO. The sperm retrieval rate was high in cases of no staining for GDNF and intense staining for SCF.

Keywords: GDNF; inhibin; microdissection TESE; SCF; Sertoli cell-only syndrome.
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