Skip Navigation



Hum. Reprod. Advance Access published online on July 8, 2005
Human Reproduction, doi:10.1093/humrep/dei132
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/10/2757    most recent
dei132v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wycherley, G.
Right arrow Articles by Hynes, A.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wycherley, G.
Right arrow Articles by Hynes, A.C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Received November 1, 2004
Revised April 19, 2005
Accepted May 10, 2005

Article

Oxidative phosphorylation and the tricarboxylic acid cycle are essential for normal development of mouse ovarian follicles

G. Wycherley 1, M.T. Kane 1, and A.C. Hynes 1*

1 Department of Physiology, National University of Ireland, Galway, University Road, Galway, Ireland

* To whom correspondence should be addressed.
A.C. Hynes, E-mail: ailish.hynes{at}nuigalway.ie


  Abstract

BACKGROUND: Mouse ovarian follicles are typically grown in upright drops of culture medium. Recently we found that culture of follicles at the medium-gas interface in inverted drops markedly improved follicular development, possibly due to improved access of oxygen to the follicle. In this study, we examined the importance of aerobic energy metabolism for follicle development by culturing mouse follicles (198 ± 16.5 initial µm diameter, mean ± SD) in the presence of phosphorylation and tricarboxylic acid (TCA) cycle inhibitors. METHODS: All inhibitors were tested in the inverted system using 100 µl medium drops in 96-well plates; certain inhibitors were also tested in upright drops with or without an oil overlay. RESULTS: The oxidative phosphorylation inhibitor rotenone (0.1, 0.5 and 1 µmol/l) totally abolished follicle growth in the inverted system; cyanide (1 mmol/l) totally abolished growth in the upright with oil system but not in the inverted system (possibly due to loss of cyanide gas due to the absence of an oil overlay). The mitochondrial uncoupler 2,4-dinitrophenol (0.5 and 1 mmol/l) also abolished growth in the inverted system. The TCA cycle inhibitor monofluoroacetate (10 mmol/l), significantly inhibited growth in all three culture systems (P < 0.01) but malonate (10 mmol/l) had no effect. CONCLUSIONS: Aerobic metabolism and an adequate oxygen supply are essential for normal follicular development.

Keywords: culture/glycolysis/inhibitors/mouse follicles/oxidative phosphorylation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
J. Ramalho-Santos, S. Varum, S. Amaral, P. C. Mota, A. P. Sousa, and A. Amaral
Mitochondrial functionality in reproduction: from gonads and gametes to embryos and embryonic stem cells
Hum. Reprod. Update, September 1, 2009; 15(5): 553 - 572.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S. E Harris, I. Adriaens, H. J Leese, R. G Gosden, and H. M Picton
Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro
Reproduction, September 1, 2007; 134(3): 415 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. T. Pagnamenta, J.-W. Taanman, C. J. Wilson, N. E. Anderson, R. Marotta, A. J. Duncan, M. B. Glindzicz, R. W. Taylor, A. Laskowski, D. R. Thorburn, et al.
Dominant inheritance of premature ovarian failure associated with mutant mitochondrial DNA polymerase gamma
Hum. Reprod., October 1, 2006; 21(10): 2467 - 2473.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.