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Hum. Reprod. Advance Access published online on June 24, 2005
Human Reproduction, doi:10.1093/humrep/dei162
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Received May 12, 2004
Revised May 11, 2005
Accepted May 20, 2005

Article

Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum

Yuki Akayama 1, Shigeki Takekida 1, Noriyuki Ohara 1*, Hisashi Tateiwa 1, Wei Chen 1, Koji Nakabayashi 1, and Takeshi Maruo 1

1 Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-Cho, Chuo-Ku, Kobe, 650-0017, Japan

* To whom correspondence should be addressed.
Noriyuki Ohara, E-mail: oharan{at}med.kobe-u.ac.jp


  Abstract

BACKGROUND: The objective of this study was to elucidate gene expression and immunolocalization of heparinbinding epidermal growth factor-like growth factor (HB-EGF) and human epidermal growth factor receptor (HER) family in the human ovary during luteal growth and regression. METHODS: Ovaries obtained from pre-menopausal women were used for immunohistochemistry and semiquantitative RT-PCR analysis. RESULTS: Immunoreactive HB-EGF was not detected in follicles or oocyte, while HB-EGF became apparent in granulosa luteal cells in the early luteal phase, and most abundant in the mid-luteal phase, but less abundant in the late luteal phase. Immunostaining for HER1 was very weak in granulosa luteal cells in the early and mid-luteal phases, and was not detected in the late luteal phase. Immunoreactive HER4 was abundant in the early luteal phase and became less abundant in the mid-luteal phase, whereas it was negative in the late luteal phase. Semiquantitative RT-PCR analysis revealed that HB-EGF and HER1 mRNA levels were high in the mid-luteal phase, whereas HER4 mRNA expression was high in the early luteal phase. CONCLUSIONS: HB-EGF may play a vital role in regulating luteal growth in a juxtacrine manner and through activating HER4 signalling.

Keywords: corpus luteum/granulosa luteal cells/heparin-binding epidermal growth factor-like growth factor (HB-EGF)/human epidermal growth factor receptor (HER).
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