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Hum. Reprod. Advance Access published online on September 19, 2005
Human Reproduction, doi:10.1093/humrep/dei265
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Received April 22, 2005
Revised July 9, 2005
Accepted July 19, 2005

Article

Cortical granules behave differently in mouse oocytes matured under different conditions

Xin-Yong Liu 1, Suo-Feng Mal 1, De-Qiang Miao 1, Dong-Jun Liu 2, Shorgan Bao 2, and Jing-He Tan 1*

1 Laboratory for Animal Reproduction and Embryology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, Shandong Province, People’s Republic of China
2 Research Center for Laboratory Animal Science, Inner Mongolia University, Huhhot 010021

* To whom correspondence should be addressed.
Jing-He Tan, E-mail: tanjh{at}sdau.edu.cn


  Abstract

BACKGROUND: To better understand the differences between in vivo (IVO) and in vitro (IVM) matured oocytes, we studied the chronological changes in cortical granule (CG) distribution and nuclear progression during maturation, and the competence of CG release and embryo development of mouse oocytes matured under different conditions. METHODS: Oocytes matured in vivo or in different culture media were used and CG distribution and release were assessed by fluorescein isothiocyanate-labelled Lens culinaris agglutinin and laser confocal microscopy. RESULTS: Tempos of nuclear maturation and CG redistribution were slower, and competence for CG exocytosis, cleavage and blastulation were lower in the IVM oocytes than in the IVO oocytes. These parameters also differed among oocytes matured in different culture media. Hypoxanthine (HX, 4 mM) blocked germinal vesicle breakdown (GVBD), postponed CG migration and prevented CG-free domain (CGFD) formation. Cycloheximide (CHX) facilitated both GVBD and CG migration, but inhibited CGFD formation. The presence of serum in maturation media enhanced CG release after aging or activation of oocytes. Maintenance of germinal vesicle intact for some time by a trace amount (0.18 mM) of HX was beneficial to oocyte cytoplasmic maturation. CONCLUSION: CGs behaved differently in mouse oocytes matured under different conditions, and cytoplasmic maturity was not fully achieved in the IVM oocytes.

Keywords: activation/cortical granule/in vitro maturation/mouse oocyte.
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