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Hum. Reprod. Advance Access published online on August 25, 2005
Human Reproduction, doi:10.1093/humrep/dei266
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Received January 18, 2005
Revised June 6, 2005
Accepted June 16, 2005

Article

Potential adverse effect of sperm DNA damage on embryo quality after ICSI

Armand Zini 1*, James Meriano 2, Karim Kader 1, Keith Jarvi 1, Carl A.Laskin 3, and Kenneth Cadesky 3

1 Division of Urology, Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada
2 LifeQuest Centre for Reproductive Medicine, University of Toronto, Toronto, Ontario, Canada
3 LifeQuest Centre for Reproductive Medicine, University of Toronto, Toronto, Ontario, Canada; Departments of Medicine, Immunology, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

* To whom correspondence should be addressed.
Armand Zini, E-mail: armand.zini{at}ssss.gouv.qc.ca


  Abstract

BACKGROUND: Sperm DNA damage is prevalent amongst infertile men and has been shown to strongly impact adversely natural reproduction, intrauterine insemination-assisted reproduction and to a lesser degree IVF/ICSI fertilization. The objective of this study was to examine further the relationship between sperm DNA denaturation (DD) and reproductive outcomes after ICSI. METHODS: We evaluated infertile couples (n = 60) undergoing IVF/ICSI at a single centre. Sperm DD was assessed by flow cytometry analysis of Acridine Orange-treated sperm and expressed as the percentage of sperm with DD. Couples were sub-grouped according to sperm DD results: group 1: 0-15%; group 2: >15-30%; group 3: >30%. RESULTS: There were no differences between the three groups with regard to maternal age, sperm parameters, oocyte maturation, fertilization or pregnancy rates. Group 3 had a significantly higher rate of multinucleation among the embryo cohorts compared to either groups 1 or 2 (20% versus 10% and 8% respectively, P = 0.04). There was a statistically insignificant trend toward an increased spontaneous pregnancy loss rate in group 3 (P =0.50). CONCLUSION: Although we did not observe significant relationships between sperm DNA damage and either fertilization or pregnancy rates, the potential adverse effect of sperm DNA damage on embryo quality and spontaneous pregnancy loss is concerning.

Keywords: DNA damage/embryo/male infertility/multinucleation/sperm.
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