Skip Navigation



Hum. Reprod. Advance Access published online on October 6, 2005
Human Reproduction, doi:10.1093/humrep/dei313
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/1/68    most recent
dei313v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dolatshad, H.
Right arrow Articles by Johnson, M.H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dolatshad, H.
Right arrow Articles by Johnson, M.H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received July 6, 2005
Revised August 12, 2005
Accepted August 26, 2005

Article

Developmental and reproductive performance in circadian mutant mice

H. Dolatshad 1, E.A. Campbell 1, L. O’Hara 1, E.S. Maywood 2, M.H. Hastings 2, and M.H. Johnson 1*

1 Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
2 MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

* To whom correspondence should be addressed.
M.H. Johnson, E-mail: mhj{at}mole.bio.cam.ac.uk


  Abstract

BACKGROUND: Genes underlying circadian rhythm generation are expressed in many tissues. We explore a role for circadian rhythms in the timing and efficacy of mouse reproduction and development using a genetic approach. METHODS: We compare fecundity in Clock{Delta}19 mutant mice (a dominant-negative protein essential for circadian rhythm activity) and in Vipr2-/- null mutant mice (affecting the generation and output of the circadian rhythm of the hypotha-lamic suprachiasmatic nucleus) with wild type (WT) litter mates under both a 12 h:12 h light:dark cycle and continuous darkness. RESULTS: Uteri from Clock{Delta}19 mice show no circadian rhythm and Vipr2-/- mice show a phase-advanced rhythm compared to WT uteri. In neither mutant line were homozygous or heterozygous fetuses lethal. Sexually mature adults of both mutant lines showed mildly reduced male in vivo (but not in vitro) fertility and irregular estrous cycles exacerbated by continuous darkness. However, pregnancy rates and neonatal litter sizes were not affected. The Clock{Delta}19mutant line was distinguishable from the Vipr2-/- null mutant line in showing more peri-natal delivery problems and very poor survival of offspring to weaning. CONCLUSIONS: A fully functional central and peripheral circadian clock is not essential for reproduction and development to term, but has critical roles peri-natally and post-partum.

Keywords: circadian rhythm/clock mutants/fecundity/mice/uterus.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Biol RhythmsHome page
L. Marpegan, T. J. Krall, and E. D. Herzog
Vasoactive Intestinal Polypeptide Entrains Circadian Rhythms in Astrocytes
J Biol Rhythms, April 1, 2009; 24(2): 135 - 143.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
C. K. Ratajczak, K. L. Boehle, and L. J. Muglia
Impaired Steroidogenesis and Implantation Failure in Bmal1-/- Mice
Endocrinology, April 1, 2009; 150(4): 1879 - 1885.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. J. Nakamura, M. T. Sellix, M. Menaker, and G. D. Block
Estrogen directly modulates circadian rhythms of PER2 expression in the uterus
Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1025 - E1031.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
V. Pilorz and S. Steinlechner
Low reproductive success in Per1 and Per2 mutant mouse females due to accelerated ageing?
Reproduction, April 1, 2008; 135(4): 559 - 568.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. S. Maywood, J. S. O'Neill, J. E. Chesham, and M. H. Hastings
Minireview: The Circadian Clockwork of the Suprachiasmatic Nuclei Analysis of a Cellular Oscillator that Drives Endocrine Rhythms
Endocrinology, December 1, 2007; 148(12): 5624 - 5634.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
M. Hastings, J. S O'Neill, and E. S Maywood
Circadian clocks: regulators of endocrine and metabolic rhythms
J. Endocrinol., November 1, 2007; 195(2): 187 - 198.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
P.-J. He, M. Hirata, N. Yamauchi, and M.-a. Hattori
Up-regulation of Per1 expression by estradiol and progesterone in the rat uterus
J. Endocrinol., September 1, 2007; 194(3): 511 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
P.-J. He, M. Hirata, N. Yamauchi, S. Hashimoto, and M.-a. Hattori
The disruption of circadian clockwork in differentiating cells from rat reproductive tissues as identified by in vitro real-time monitoring system
J. Endocrinol., June 1, 2007; 193(3): 413 - 420.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
E. S. Maywood, J. S. O'Neill, A. B. Reddy, J. E. Chesham, H. M. Prosser, C. P. Kyriacou, S. I. H. Godinho, P. M. Nolan, and M. H. Hastings
Genetic and Molecular Analysis of the Central and Peripheral Circadian Clockwork of Mice
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 85 - 94.
[Abstract] [PDF]

Home page
 Hum ReprodHome page
E.A. Campbell, L. O'Hara, R.D. Catalano, A.M. Sharkey, T.C. Freeman, and M. H. Johnson
Temporal expression profiling of the uterine luminal epithelium of the pseudo-pregnant mouse suggests receptivity to the fertilized egg is associated with complex transcriptional changes
Hum. Reprod., October 1, 2006; 21(10): 2495 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. J. Kennaway, J. A. Owens, A. Voultsios, and T. J. Varcoe
Functional central rhythmicity and light entrainment, but not liver and muscle rhythmicity, are Clock independent
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1172 - R1180.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. N. Karman and S. A. Tischkau
Circadian Clock Gene Expression in the Ovary: Effects of Luteinizing Hormone
Biol Reprod, October 1, 2006; 75(4): 624 - 632.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. J Boden and D. J Kennaway
Circadian rhythms and reproduction.
Reproduction, September 1, 2006; 132(3): 379 - 392.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Fahrenkrug, B. Georg, J. Hannibal, P. Hindersson, and S. Gras
Diurnal Rhythmicity of the Clock Genes Per1 and Per2 in the Rat Ovary
Endocrinology, August 1, 2006; 147(8): 3769 - 3776.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.