Skip Navigation



Hum. Reprod. Advance Access published online on October 6, 2005
Human Reproduction, doi:10.1093/humrep/dei315
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/2/421    most recent
dei315v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cao, W.G.
Right arrow Articles by Akoum, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cao, W.G.
Right arrow Articles by Akoum, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received November 12, 2004
Revised August 2, 2005
Accepted August 22, 2005

Article

Tumour necrosis factor-{alpha} up-regulates macrophage migration inhibitory factor expression in endometrial stromal cells via the nuclear transcription factor NF-{kappa}B

W.G. Cao 1, M. Morin 1, V. Sengers 1, C. Metz 2, T. Roger 3, R. Maheux 1, and A. Akoum 1*

1 Unité d’endocrinologie de la reproduction, Centre de Recherche, Hôpital Saint-François d’Assise, Centre Hospitalier Universitaire de Québec, Faculté de Médecine, Université Laval, Québec, Canada
2 Institute for Medical Research at North Shore-LIJ, NY, USA
3 Service des Maladies Infectieuses, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

* To whom correspondence should be addressed.
A. Akoum, E-mail: ali.akoum{at}crsfa.ulaval.ca


  Abstract

BACKGROUND: A series of controlled changes including proliferation, secretion and menstrual shedding occur in the human endometrium during every normal menstrual cycle. Macrophage migration inhibitory factor (MIF), a multifunctional cytokine with numerous proinflammatory, immunomodulatory and angiogenic properties, appears to be expressed in the human endometrium and to follow a regulated cycle phase-dependent expression, but the mechanisms underlying endometrial MIF expression remain to be fully elucidated. METHODS AND RESULTS: Results from enzyme-linked immunosorbent assay (ELISA) demonstrated a significant dose- and time-dependent increase in MIF secretion by human endometrial cells in response to tumour necrosis factor-alpha (TNF-{alpha}) (0.1-100 ng/ml). This increase was also observed at the mRNA level as shown by reverse transcription (RT)-PCR. Curcumin (10-8 mol/l), a known nuclear factor (NF)-{kappa}B inhibitor, inhibited the TNF-{alpha}-induced pI{kappa}B phosphorylation as shown by western blotting, NF-{kappa}B translocation into the nucleus as shown by electrophoretic mobility shift assay, and MIF synthesis and secretion as measured by ELISA and RT-PCR. The expression of a dominant-negative NF-{kappa}B inhibitor (I{kappa}B) significantly decreased the TNF-{alpha}-induced MIF promoter activity as analysed by transient cell transfection. CONCLUSIONS: These results indicate clearly that TNF-{alpha} up-regulates the expression of MIF in endometrial stromal cells. This took place possibly through NF-{kappa}B activation, and may play an important role in the physiology of the human endometrium.

Keywords: endometrium/MIF/NF-{kappa}B/TNF-{alpha}.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
E. M. C. Ohlsson Teague, C. G. Print, and M. L. Hull
The role of microRNAs in endometriosis and associated reproductive conditions
Hum. Reprod. Update, September 22, 2009; (2009) dmp034v1.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
Z. Z. Zhao, D. R. Nyholt, L. Le, S. Thomas, C. Engwerda, L. Randall, S. A. Treloar, and G. W. Montgomery
Genetic variation in tumour necrosis factor and lymphotoxin is not associated with endometriosis in an Australian sample
Hum. Reprod., September 1, 2007; 22(9): 2389 - 2397.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R. Gonzalez-Ramos, J. Donnez, S. Defrere, I. Leclercq, J. Squifflet, J.-C. Lousse, and A. Van Langendonckt
Nuclear factor-kappa B is constitutively activated in peritoneal endometriosis
Mol. Hum. Reprod., July 1, 2007; 13(7): 503 - 509.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Arcuri, L. Buchwalder, P. Toti, M. Cintorino, P. Tosi, C. J. Lockwood, B. Rybalov, and F. Schatz
Differential Regulation of Colony Stimulating Factor 1 and Macrophage Migration Inhibitory Factor Expression by Inflammatory Cytokines in Term Human Decidua: Implications for Macrophage Trafficking at the Fetal-Maternal Interface
Biol Reprod, March 1, 2007; 76(3): 433 - 439.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.