Skip Navigation


Hum. Reprod. Advance Access first published online on November 10, 2005
This version published online on December 14, 2005
Human Reproduction, doi:10.1093/humrep/dei345
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/2/503    most recent
dei345v2
dei345v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mateizel, I.
Right arrow Articles by Steirteghem, A. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mateizel, I.
Right arrow Articles by Steirteghem, A. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received June 6, 2005
Revised August 8, 2005
Accepted August 25, 2005

Article

Derivation of human embryonic stem cell lines from embryos obtained after IVF and after PGD for monogenic disorders

I. Mateizel 1 *, N. De Temmerman 1 *, U. Ullmann 1 *, G. Cauffman 1, K. Sermon 2 *, H. Van de Velde 3, M. De Rycke 2, E. Degreef 4, P. Devroey 3, I. Liebaers 2, and A. Van Steirteghem 3

1 Research Centre for Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium
2 Research Centre for Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium; Centre for Medical Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium
3 Research Centre for Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium; Centre for Reproductive Medicine, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium
4 Department of Pathology, University Hospital and Medical School of the Vrije Universiteit Brussel (Dutch-speaking Brussels Free University) Laarbeeklaan 101, 1090 Brussels, Belgium

* To whom correspondence should be addressed.
K. Sermon, E-mail: karen.sermon{at}az.vub.ac.be


  Abstract

BACKGROUND: Human embryonic stem (hES) cells are pluripotent cells usually derived from the inner cell mass (ICM) of blastocysts. Because of their ability to differentiate into all three embryonic germ layers, hES cells represent an important material for studying developmental biology and cell replacement therapy. hES cell lines derived from blastocysts diagnosed as carrying a genetic disorder after PGD represent in vitro disease models. METHODS: ICMs isolated by immunosurgery from human blastocysts donated for research after IVF cycles and after PGD were plated in serum-free medium (except VUB01) on mouse feeder layers. RESULTS: Five hES cell lines were isolated, two from IVF embryos and three from PGD embryos. All lines behave similarly in culture and present a normal karyotype. The lines express all the markers considered characteristic of undifferentiated hES cells and were proven to be pluripotent both in vitro and in vivo (ongoing for VUB05_HD). CONCLUSIONS: We report here on the derivation of two hES cell lines presumed to be genetically normal (VUB01 and VUB02) and three hES cell lines carrying mutations for myotonic dystrophy type 1 (VUB03_DM1), cystic fibrosis (VUB04_CF) and Huntington disease (VUB05_HD).

Keywords: cystic fibrosis/disease models/human embryonic stem cells/Huntington disease/myotonic dystrophy.

*These authors contributed equally to this work


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
M. Geens, I. Mateizel, K. Sermon, M. De Rycke, C. Spits, G. Cauffman, P. Devroey, H. Tournaye, I. Liebaers, and H. Van de Velde
Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos
Hum. Reprod., November 1, 2009; 24(11): 2709 - 2717.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I. Mateizel, C. Spits, A. Verloes, A. Mertzanidou, I. Liebaers, and K. Sermon
Characterization of CD30 expression in human embryonic stem cell lines cultured in serum-free media and passaged mechanically
Hum. Reprod., October 1, 2009; 24(10): 2477 - 2489.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I. Liebaers, S. Desmyttere, W. Verpoest, M. De Rycke, C. Staessen, K. Sermon, P. Devroey, P. Haentjens, and M. Bonduelle
Report on a consecutive series of 581 children born after blastomere biopsy for preimplantation genetic diagnosis
Hum. Reprod., August 27, 2009; (2009) dep298v1.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K.D. Sermon, C. Simon, P. Braude, S. Viville, J. Borstlap, and A. Veiga
Creation of a registry for human embryonic stem cells carrying an inherited defect: joint collaboration between ESHRE and hESCreg
Hum. Reprod., July 1, 2009; 24(7): 1556 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H.-F. Chen, H.-C. Kuo, W. Chen, F.-C. Wu, Y.-S. Yang, and H.-N. Ho
A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals
Hum. Reprod., January 1, 2009; 24(1): 71 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Cauffman, M. De Rycke, K. Sermon, I. Liebaers, and H. Van de Velde
Markers that define stemness in ESC are unable to identify the totipotent cells in human preimplantation embryos
Hum. Reprod., January 1, 2009; 24(1): 63 - 70.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
A. Raya, I. Rodriguez-Piza, B. Aran, A. Consiglio, P.N. Barri, A. Veiga, and J.C. Izpisua Belmonte
Generation of Cardiomyocytes from New Human Embryonic Stem Cell Lines Derived from Poor-quality Blastocysts
Cold Spring Harb Symp Quant Biol, November 26, 2008; (2008) sqb.2008.73.038v2.
[Abstract] [PDF]

Home page
 Hum ReprodHome page
X. Sun, X. Long, Y. Yin, Y. Jiang, X. Chen, W. Liu, W. Zhang, H. Du, S. Li, Y. Zheng, et al.
Similar biological characteristics of human embryonic stem cell lines with normal and abnormal karyotypes
Hum. Reprod., October 1, 2008; 23(10): 2185 - 2193.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop
Stem Cells and Cell Therapies in Lung Biology and Lung Diseases
Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667.
[Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
N. De Temmerman, S. Seneca, A. Van Steirteghem, P. Haentjens, J. Van der Elst, I. Liebaers, and K.D. Sermon
CTG repeat instability in a human embryonic stem cell line carrying the myotonic dystrophy type 1 mutation
Mol. Hum. Reprod., July 1, 2008; 14(7): 405 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
U. Ullmann, C. Gilles, M. De Rycke, H. Van de Velde, K. Sermon, and I. Liebaers
GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions
Mol. Hum. Reprod., March 1, 2008; 14(3): 169 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
T. Turetsky, E. Aizenman, Y. Gil, N. Weinberg, Y. Shufaro, A. Revel, N. Laufer, A. Simon, D. Abeliovich, and B.E. Reubinoff
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis
Hum. Reprod., January 1, 2008; 23(1): 46 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
U. Ullmann, P. In't Veld, C. Gilles, K. Sermon, M. De Rycke, H. Van de Velde, A. Van Steirteghem, and I. Liebaers
Epithelial-mesenchymal transition process in human embryonic stem cells cultured in feeder-free conditions
Mol. Hum. Reprod., January 1, 2007; 13(1): 21 - 32.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
H. Skottman and O. Hovatta
Culture conditions for human embryonic stem cells.
Reproduction, November 1, 2006; 132(5): 691 - 698.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.