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Hum. Reprod. Advance Access published online on October 27, 2005
Human Reproduction, doi:10.1093/humrep/dei375
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received July 5, 2005
Revised September 2, 2005
Accepted September 21, 2005

Article

Tumour necrosis factor-{alpha}, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model*

Sophie Debrock 1*, Bart De Strooper 2, Sarah Vander Perre 1, Joseph A. Hill 3, and Thomas M D’Hooghe 1

1 Leuven University Fertility Center, UZ Gasthuisberg, KU Leuven, Belgium
2 Center for Human Genetics KU Leuven, Belgium
3 The Fertility Centers of New England, Reading, MA, USA

* To whom correspondence should be addressed.
Sophie Debrock, E-mail: sophie.debrock{at}uz.kuleuven.ac.be


  Abstract

BACKGROUND: A key factor in the pathogenesis of endometriosis is the endometrial-peritoneal adhesion. To study the pathogenesis of endometriosis, a quantitative in vitro assay (QIVA) was developed to measure in vitro adhesion between human endometrial epithelial cells and mesothelial cells using commercially available cell lines. Using the QIVA, the hypothesis was tested that tumour necrosis factor-{alpha} (TNF-{alpha}), interleukin-6 (IL-6) and interleukin-8 (IL-8) promote adhesion of endometrial epithelial cells to mesothelial cells. METHODS: Mesothelial cells were pre-treated with TNF-{alpha}, IL-6 or IL-8 in various concentrations (ranging from 0 to 1000 IU/ml) for 24 h. Confluent endometrial epithelial cells were labelled with [35S]methionine, added to the confluent mesothelial cells and incubated for 1 h. After incubation, non-adhering cells were removed and adherent cells were solubilized and their [35S]methionine radioactivity was counted to quantify the adherence of endometrial epithelial cells to mesothelial cells. RESULTS: The in vitro adhesion of human endometrial epithelial cells to human mesothelial cells was inhibited in a dose-dependent manner by TNF-{alpha} (P = 0.0007), IL-6 (P < 0.0001) and IL-8 (P = 0.0004). CONCLUSIONS: Using a quantitative in vitro adhesion assay, we were unable to confirm our hypothesis that TNF-{alpha}, IL-6 and IL-8 promote the in vitro adhesion between endometrial epithelial cells and mesothelial cells.

Keywords: cytokines/endometriosis/endometrium/in vitro adhesion assay/mesothelium.
*In part presented at the VII World Congress on Endometriosis, San Diego, California, February 24-27, 2002 and at the 58th Annual Meeting of the ASRM Conference, Seattle, Washington, October 12-17, 2002.
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