Skip Navigation



Hum. Reprod. Advance Access published online on February 23, 2006
Human Reproduction, doi:10.1093/humrep/del018
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrowOA All Versions of this Article:
21/6/1564    most recent
del018v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Roulet, V.
Right arrow Articles by Dejucq-rainsford, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roulet, V.
Right arrow Articles by Dejucq-rainsford, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. for Permissions, please email: journals.permissions@oxfordjournals.org The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Received April 15, 2005
Revised November 29, 2005
Accepted January 10, 2006

Article

Human testis in organotypic culture: application for basic or clinical research

V. Roulet 1, H. Denis 1, C. Staub 2, A.Le Tortorec 2, B. Delaleu 3, A.P. Satie 1, J.J. Patard 4, B. Jégou 1 *, and N. Dejucq-rainsford 1

1 INSERM, U625, GERHM, Campus de Beaulieu; Université de Rennes 1; IFR 140, Rennes
2 INSERM, U625, GERHM, Campus de Beaulieu; IFR 140, Rennes
3 Service de microscopie électronique, INRA PRC, Tours
4 Service d’Urologie, CHUR Pontchaillou, Rennes, France

* To whom correspondence should be addressed.
B. Jégou, E-mail: bernard.jegou{at}rennes.inserm.fr


  Abstract

BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2'-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue.

Keywords: human/organotypic culture/testis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
A. Le Tortorec, H. Denis, A-P. Satie, J-J. Patard, A. Ruffault, B. Jegou, and N. Dejucq-Rainsford
Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation
Hum. Reprod., September 1, 2008; 23(9): 2095 - 2103.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Jahnukainen, J. Ehmcke, M. Nurmio, and S. Schlatt
Irradiation Causes Acute and Long-Term Spermatogonial Depletion in Cultured and Xenotransplanted Testicular Tissue from Juvenile Nonhuman Primates
Endocrinology, November 1, 2007; 148(11): 5541 - 5548.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P. A. Fowler, D. R. Abramovich, N. E. Haites, P. Cash, N. P. Groome, A. Al-Qahtani, T. J. Murray, and R. G. Lea
Human fetal testis Leydig cell disruption by exposure to the pesticide dieldrin at low concentrations
Hum. Reprod., November 1, 2007; 22(11): 2919 - 2927.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. Bujan, M. Daudin, N. Moinard, P. Plante, J. Parinaud, and C. Pasquier
Azoospermic HIV-1 infected patients wishing to have children: proposed strategy to reduce HIV-1 transmission risk during sperm retrieval and intracytoplasmic sperm injection: Case Report
Hum. Reprod., September 1, 2007; 22(9): 2377 - 2381.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
V. Roulet, A.-P. Satie, A. Ruffault, A. L. Tortorec, H. Denis, O. Guist'hau, J.-J. Patard, N. Rioux-Leclerq, J. Gicquel, B. Jegou, et al.
Susceptibility of Human Testis to Human Immunodeficiency Virus-1 Infection in Situ and in Vitro
Am. J. Pathol., December 1, 2006; 169(6): 2094 - 2103.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.