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Hum. Reprod. Advance Access published online on February 24, 2006
Human Reproduction, doi:10.1093/humrep/del036
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received November 29, 2005
Revised January 6, 2006
Accepted January 19, 2006

Article

The miscarriage-associated HLA-G -725G allele influences transcription rates in JEG-3 cells

C. Ober 1 *, C. Billstrand 2, S. Kuldanek 2, and Z. Tan 2

1 Department of Human Genetics; Department of Obstetrics and Gynecology, The University of Chicago, Chicago, IL, USA
2 Department of Human Genetics, The University of Chicago, Chicago, IL, USA

* To whom correspondence should be addressed.
C. Ober, E-mail: c-ober{at}genetics.uchicago.edu


  Abstract

BACKGROUND: HLA-G is a non-classical HLA with important immunomodulatory roles in pregnancy. A polymorphism in the promoter region, -725G, was previously associated with sporadic miscarriage in women who were unselected with respect to reproductive history. In this study, the transcription levels of different HLA-G promoter haplotypes were examined to determine whether the miscarriage-associated -725G allele influences transcription. METHODS: Five naturally occurring promoter haplotypes and three variant haplotypes created by site-directed mutagenesis were sub-cloned into luciferase expression vectors and transfected into JEG-3 cells. Expression levels of these eight haplotypes were examined in cultured cells before and after treatment with inter-feron-{beta} (IFN-{beta}), cytosine-5-DNA methyltransferase (M. SssI) and 5-aza-2'-deoxycytidine. Differences in expression levels between haplotypes were determined by analysis of variance (ANOVA). RESULT: Promoter haplotypes with the miscarriage-associated -725G allele were expressed at significantly higher levels in all culture conditions compared with otherwise identical haplotypes that had a -725C or -725T allele. CONCLUSION: Variation in the HLA-G promoter region influences transcription rates. Contrary to expectations, increased expression of HLA-G may be disadvantageous in some pregnancies.

Keywords: gene expression/HLA-G/methylation/miscarriage/polymorphisms.
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[Abstract] [Full Text] [PDF]


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