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Hum. Reprod. Advance Access published online on February 22, 2006
Human Reproduction, doi:10.1093/humrep/del041
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 23, 2005
Revised January 23, 2006
Accepted January 25, 2006

Article

Blastocyst development after assisted reproduction using spermatozoa obtained after testicular stem cell transplantation in mice

E. Goossens 1 *, V. Frederickx 1, G.De Block 1, A.Van Steirteghem 1, and H. Tournaye 1

1 Research Laboratory for Reproduction and Genetics (EMGE), Dutch-speaking Brussels Free University, Brussels, Belgium

* To whom correspondence should be addressed.
E. Goossens, E-mail: ellen.goossens{at}az.vub.ac.be


  Abstract

BACKGROUND: Since its introduction in 1994, testicular stem cell transplantation (TSCT) has been widely used for research. This technique may also become important for preserving fertility in pre-pubertal cancer patients. Therefore, it is necessary to investigate the safety aspects of reproduction using spermatozoa obtained after TSCT. In this study, preimplantation development of mouse embryos, using spermatozoa obtained after TSCT, was examined. METHODS: TSCT-derived spermatozoa were used for IVF and ICSI. Embryos were cultured for five days until they reached blastocyst stage and were evaluated by differential staining. RESULTS: IVF revealed significantly lower fertilization and development rates after TSCT-IVF compared to control-IVF. Blastocysts derived from TSCT-IVF had significantly lower inner cell mass numbers (ICMs) and lower ICM/trophectoderm (TE) ratios compared to control-IVF blastocysts. No differences in fertilization and development rates were observed between TSCT-ICSI and control-ICSI, and blastocyst quality in the transplanted group was similar to that of the control blastocysts. CONCLUSION: Our study showed that after TSCT-IVF, fertilization and preimplantation development were disturbed and blastocysts showed reduced ICM and ICM/TE ratio. However, after TSCT-ICSI, both fertilization and preimplantation development were normal and blastocyst formation was comparable to control-ICSI.

Keywords: assisted reproduction/blastocyst/stem cell/testis/transplantation.
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