Hum. Reprod. Advance Access published online on March 20, 2006
Human Reproduction, doi:10.1093/humrep/del074
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1 Department of Biomedical Sciences and Technologies
* To whom correspondence should be addressed. BACKGROUND: To ascertain whether different hormonal treatment protocols could affect metaphase II (MII) spindle morphology, meiotic spindle organization was detected in prepubertal mouse oocytes matured under conditions allowing spontaneous, FSH- or epidermal growth factor (EGF)-dependent meiotic maturation. METHODS: Oocyte-cumulus complexes (OCCs) were matured either spontaneously (control; n = 270) or in the presence of hypoxanthine (Hx) plus FSH (n = 400) or EGF (n = 370). Spindles were detected by immunofluorescence analysis. In vivo ovulated (IVO) oocytes were processed similarly. RESULTS: IVO oocytes displayed spindles underlying the oolemma and with focused poles marked by spots of
Received December 23, 2005
Revised February 13, 2006
Accepted February 17, 2006
Article
Meiotic spindle configuration is differentially influenced by FSH and epidermal growth factor during in vitro maturation of mouse oocytes
G. Rossi 1,
G. Macchiarelli 2,
M.G. Palmerini 2,
R. Canipari 3,
and
S. Cecconi 4 *
2 Department of Experimental Medicine, University of L’Aquila, L’Aquila
3 Department of Histology and Medical Embryology, University of Roma, ‘La Sapienza’, Roma, Italy
4 Department of Biomedical Sciences and Technologies, University of L’Aquila, Via Vetoio, Loc. Coppito, 67010 Coppito, L’Aquila, Italy
S. Cecconi, E-mail: cecconi{at}univaq.it
Abstract 
-tubulin, whereas the majority (89%) of control oocytes had barrel-shaped spindles, positioned away from the oolemma, and with -tubulin distributed along microtubules. Similar configuration/localization was found in 85% of the oocytes matured in vitro in the presence of Hx and FSH. In the presence of Hx-EGF, 35% of the oocytes showed spindles with an IVO-like configuration, although -tubulin was homogeneously distributed throughout microtubules. Independently of spindle shape, 52% of EGF-stimulated oocytes had spindles positioned near the oolemma, in comparison to just 24% of FSH-treated and 13% of control oocytes. CONCLUSIONS: These results indicate that FSH and EGF can differently affect meiotic spindle morphology, and that EGF might be a stronger contributor than FSH to the acquisition of oocyte competence.
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