Skip Navigation



Hum. Reprod. Advance Access published online on June 30, 2006
Human Reproduction, doi:10.1093/humrep/del093
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/10/2514    most recent
del093v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Panzan, M. Q.
Right arrow Articles by Baracat, E. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Panzan, M. Q.
Right arrow Articles by Baracat, E. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 13, 2005
Revised February 24, 2006
Accepted March 3, 2006

Article

Metoclopramide-induced hyperprolactinaemia caused marked decline in pinopodes and pregnancy rates in mice

Michele Quarante Panzan 1 *, José Maria Soares Júnior 1 *, Eduardo Leme Alves da Motta 1, Edna Freymuller Haapalainen 2, Manuel de Jesus Simões 2, Heloisa Allegro Baptista 3, Mauro Abi Haidar 1, and Edmund C. Baracat 1 *

1 Department of Gynecology, São Paulo, Brazil
2 Department of Morphology, São Paulo, Brazil
3 CEDEME, The Federal University of São Paulo, São Paulo, Brazil

* To whom correspondence should be addressed.
Edmund C. Baracat, E-mail: baracat.gineco{at}epm.br


  Abstract

BACKGROUND: The impact of hyperprolactinaemia on endometrial function, along with embryo implantation, has been the subject of discussion. This article examines whether experimental hyperprolactinaemia can affect mouse ovarian function, endometrial pinopodes and embryo implantation. METHODS: For pinopode analysis, 60 female mice were randomly divided into two groups: control (vehicle) and experimental [metoclopramide (MCP) 200 µg per day]. Injections were given subcutaneously for 50 days, and then, normally cycling females were housed with male mice for copulation during proestrus. The animals were killed on the fifth day following coitus when the antimes-ometrium portions of the uterine horns were removed for endometrial analysis. Blood was collected for prolactin (PRL) determination. In the second experiment, 60 female mice were used to evaluate the ovarian function by measuring estrogen and progesterone levels and counting luteal bodies and oocytes in the oviduct and uterus during estrus. RESULTS: The highest pregnancy rates and the largest population of pinopodes were both found in the vehicle group (P < 0.01). Estrogen and progesterone levels in MCP-treated mice were lower than those in control mice (P < 0.05). Also, the number of implantations was significantly lower in the MCP-treated group compared with the vehicle group after embryo transfer (P < 0.001). CONCLUSION: PRL seems to have suppressive effects on ovarian function and the number of pinopodes; conceivably, hyperprolactinaemia has a negative effect on mouse embryo implantation.

Keywords: embryo implantation/hyperprolactinaemia/metoclopramide/mice/pinopodes.

* Both authors contributed equally to this study.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
C.E. Quinn and R.F. Casper
Pinopodes: a questionable role in endometrial receptivity
Hum. Reprod. Update, March 1, 2009; 15(2): 229 - 236.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Shao, M. Nutu, B. Weijdegard, E. Egecioglu, J. Fernandez-Rodriguez, E. Tallet, V. Goffin, C. Ling, and H. Billig
Differences in Prolactin Receptor (PRLR) in Mouse and Human Fallopian Tubes: Evidence for Multiple Regulatory Mechanisms Controlling PRLR Isoform Expression in Mice
Biol Reprod, October 1, 2008; 79(4): 748 - 757.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.