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Hum. Reprod. Advance Access published online on April 6, 2006
Human Reproduction, doi:10.1093/humrep/del105
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received January 19, 2006
Revised March 1, 2006
Accepted March 14, 2006

Article

Evaluation of in vivo conception after testicular stem cell transplantation in a mouse model shows altered post-implantation development

Ellen Goossens 1 *, Veerle Frederickx 1, Gert De Block 1, André Van Steirteghem 1, and Herman Tournaye 1

1 Research Laboratory for Reproduction and Genetics (EMGE), Faculty of Medicine and Pharmacy, Dutch-speaking Brussels Free University, Brussels, Belgium

* To whom correspondence should be addressed.
Ellen Goossens, E-mail: ellen.goossens{at}az.vub.ac.be


  Abstract

BACKGROUND: Apart from research applications, testicular stem cell transplantation (TSCT) may one day also have valuable clinical applications. Therefore, it is important to investigate whether this technique is a safe method to have progeny. This controlled study aims at evaluating the fetuses and the live born offspring obtained after TSCT in male mice. METHODS: Male mice were mated with wild-type (WT) females after TSCT to produce offspring. First, fetuses were evaluated on the 17th gestational day. The length, weight and morphological age were compared to those of control mouse fetuses. The live born offspring were then investigated for their reproductive potential over three generations. RESULTS: The litter sizes after TSCT were decreased compared to controls. Fetuses showed developmental retardation of a quarter of a day, but no major external abnormalities were observed. The live born pups were able to produce normal litter sizes, at least until the third generation. CONCLUSIONS: Transplanted animals are able to reproduce naturally. Although litter sizes are lower and development is retarded, no major morphological or procreative abnormalities were observed.

Keywords: fetus/offspring/spermatogenesis/testis/transplantation.
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