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Hum. Reprod. Advance Access published online on May 9, 2006
Human Reproduction, doi:10.1093/humrep/del134
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received November 8, 2005
Revised April 4, 2006
Accepted April 7, 2006

Article

Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications

J. Erenpreiss 1 *, M. Bungum 2, M. Spano 3, S. Elzanaty 4, J. Orbidans 5, and A. Giwercman 4

1 Fertility Centre, Malmö University Hospital, Lund University, Malmö, Sweden; Andrology Laboratory, Riga Stradins University, Riga, Latvia
2 Fertility Centre, Malmö University Hospital, Lund University, Malmö, Sweden; Fertility Clinic, Viborg Hospital (Skive), Skive, Denmark
3 Section of Toxicology and Biomedical Sciences, BIOTEC-MED, ENEA CR Casaccia, Rome, Italy
4 Fertility Centre, Malmö University Hospital, Lund University, Malmö, Sweden
5 Andrology Laboratory, Riga Stradins University, Riga, Latvia

* To whom correspondence should be addressed.
J. Erenpreiss, E-mail: juris.erenpreiss{at}med.lu.se


  Abstract

BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI >30% in the first test had DFI <30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI >30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated.

Keywords: flow cytometry/infertility/SCSA/sperm chromatin/sperm DNA integrity.
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