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Hum. Reprod. Advance Access published online on July 22, 2006
Human Reproduction, doi:10.1093/humrep/del156
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received February 10, 2006
Revised April 12, 2006
Accepted April 13, 2006

Article

Reactive oxygen species level in follicular fluid--embryo quality marker in IVF?

S. Das 1, R. Chattopadhyay 2, S. Ghosh 1, S. Ghosh 2, S.K. Goswami 2, B.N. Chakravarty 2, and K. Chaudhury 1 *

1 School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India
2 Institute of Reproductive Medicine, Salt Lake, Kolkata, West Bengal, India

* To whom correspondence should be addressed.
K. Chaudhury, E-mail: koel{at}smst.iitkgp.ernet.in


  Abstract

BACKGROUND: The impact of oxidative stress in female reproduction is not clear. Contradictory reports on the effect of various oxidative stress markers on follicular fluid, oocytes and embryo quality and fertilization potential exist. The objectives of this study were to examine reactive oxygen species (ROS) levels in follicular fluid of women undergoing IVF and to relate these levels to embryo formation and quality. METHODS AND RESULTS: A total of 208 follicular fluid samples were obtained from 78 women undergoing controlled ovarian stimulation and analysed for ROS and lipid peroxidation (LPO). These samples were divided into groups I and II which represented follicular fluid containing grade III and grade II oocytes, respectively. These groups were further subdivided into groups IA, IB, IIA and IIB according to embryo quality. Subgroups IA and IIA consisted of follicular fluid samples corresponding to grade I/II embryo formation. Subgroups IB and IIB represented fertilization failure/pro-nucleolus (PN) arrest/ grade III embryos. No significant correlation was observed in ROS levels on comparing groups I and II (P > 0.05). However, ROS levels were observed to be significantly different on comparing groups IA and IB (P ≤ 0.01) and groups IIA and IIB (P ≤ 0.05). LPO levels further supported our results. CONCLUSION: ROS levels in follicular fluid appear to play a significant role in embryo formation and quality.

Keywords: embryo/follicular fluid/IVF/lipid peroxidation/reactive oxygen species.
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