Hum. Reprod. Advance Access published online on June 8, 2006
Human Reproduction, doi:10.1093/humrep/del176
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Division of Histology and Cell Biology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences; Department of Obstetrics and Gynecology, Nagasaki University School of Medicine
* To whom correspondence should be addressed. BACKGROUND: Although intraepithelial lymphocytes (IELs) in human oviductal epithelium have been implicated in the regulation of local immunity, the precise kinetics and mechanism of steroid regulation of IEL are largely unknown. METHODS: We examined the localization of estrogen receptors (ERs) and progesterone receptors (PRs) in 41 human oviducts by immunohistochemistry. These tissues were obtained from various menstrual cycles, also from both post-menopausal women and tubal pregnancies. The expressions of ER
Received March 10, 2006
Revised April 18, 2006
Accepted April 25, 2006
Article
Identification of estrogen receptor
Shirendeb Ulziibat 1,
Kuniaki Ejima 2,
Yasuaki Shibata 3,
Yoshitaka Hishikawa 2,
Michio Kitajima 4,
Akira Fujishita 4,
Tadayuki Ishimaru 5,
and
Takehiko Koji 6 *
-positive intraepithelial lymphocytes and their possible roles in normal and tubal pregnancy oviducts
2 Division of Histology and Cell Biology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences
3 Division of Oral Pathology and Bone Metabolism, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
4 Department of Obstetrics and Gynecology, Nagasaki University School of Medicine
5 Department of Obstetrics and Gynecology, Nagasaki University School of Medicine; Present address: Sasebo Chuo Hospital, Sasebo, Nagasaki, Japan
6 Division of Histology and Cell Biology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Japan
Takehiko Koji, E-mail: tkoji{at}net.nagasaki-u.ac.jp
Abstract 
mRNA and membrane (m)PR mRNA were examined by in situ hybridization and RT-PCR, respectively. RESULTS: Most of the IEL expressed ER at both mRNA and protein levels. The number of ER-positive IEL, which were identified as CD8-positive T lymphocytes and also were mPR positive, was increased in the late proliferative, the mid-secretory and late secretory phases in normally cycling women (P< 0.05). Interestingly, in tubal pregnancy, ER-positive IELs were consistently abundant. In addition, we found a high Ki-67-labelling index for IEL, although ER
was entirely absent in the tubal pregnancy oviducts. CONCLUSIONS: These results suggest that the number of IEL fluctuated because of estrogen and progesterone levels probably through ER and mPR, respectively. ER-positive IEL may be involved in regulating immune tolerance in tubal pregnancy oviducts.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Cvoro, D. Tatomer, M.-K. Tee, T. Zogovic, H. A. Harris, and D. C. Leitman Selective Estrogen Receptor- Agonists Repress Transcription of Proinflammatory Genes J. Immunol., January 1, 2008; 180(1): 630 - 636. [Abstract] [Full Text] [PDF]
|
|