Skip Navigation



Hum. Reprod. Advance Access published online on June 28, 2006
Human Reproduction, doi:10.1093/humrep/del189
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/9/2238    most recent
del189v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Koscinski, I.
Right arrow Articles by Buisine, M.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koscinski, I.
Right arrow Articles by Buisine, M.-P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received February 27, 2006
Revised April 6, 2006
Accepted April 24, 2006

Article

MUC4 gene polymorphism and expression in women with implantation failure

Isabelle Koscinski 1 *, Stephane Viville 2, Nicole Porchet 3, Annie Bernigaud 4, Fabienne Escande 4, Andre Defossez 5, and Marie-Pierre Buisine 3

1 Laboratoire de Biologie de la Reproduction, C.M.C.O., Schiltigheim Cedex, France; INSERM Unité 560, Place de Verdun, France; Laboratoire de Biologie de la Reproduction, Hôpital Jeanne de Flandre, Lille Cedex, France
2 Laboratoire de Biologie de la Reproduction, C.M.C.O., Schiltigheim Cedex, France; IGBMC, Illkirch, France
3 INSERM Unité 560, Place de Verdun, France; Laboratoire de Biochimie et Biologie Moléculaire, Parc Eurasanté, CHRU de Lille, France; Faculté de Médecine, Département de Biochimie et Biologie Moléculaire, Université de Lille II, France
4 INSERM Unité 560, Place de Verdun, France
5 Laboratoire de Biologie de la Reproduction, Hôpital Jeanne de Flandre, Lille Cedex, France; Faculté de Médecine, Laboratoire d’Histologie et d’Embryologie, Faculté de Médecine, Place de Verdun, Lille Cedex, France

* To whom correspondence should be addressed.
Isabelle Koscinski, E-mail: isabelle.koscinski{at}chru-strasbourg.fr


  Abstract

INTRODUCTION: The molecular mechanism of human embryo implantation is poorly understood. The role of MUC4 mucin, present in endometrial epithelium, has never been explored, and results obtained in animal studies strongly suggest a role in implantation. We investigated the role of MUC4 in human embryo implantation. METHODS AND RESULTS: We analysed the MUC4 variable number of tandem repeat (VNTR) polymorphism in three populations by Southern blot analysis: spontaneously fertile patients (C), infertile patients with repeated unexplained implantation failures after IVF (IF) and patients with a child after IVF (IVF-C). We found no differences in the size or allelic distribution of MUC4 VNTR between these three populations. We also examined, in IVF-C and IF groups, the endometrial expression of MUC4 mRNA as well as the expression of the MUC4 glyco-protein together with estrogen receptor (ER) and progesterone receptor (PR). No expression differences could be detected. However, we noticed a pattern of expression for MUC4 protein, which is limited to patches of cells in the luminal and glandular epithelium. CONCLUSIONS: We conclude that the different-sized MUC4 alleles do not interfere with implantation. The absence of coexpression of MUC4 and the steroid receptors suggests that MUC4 expression is not directly regulated by steroids.

Keywords: endometrium/implantation failure/infertility polymorphism/MUC4.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
P. Chaturvedi, A. P. Singh, and S. K. Batra
Structure, evolution, and biology of the MUC4 mucin
FASEB J, April 1, 2008; 22(4): 966 - 981.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.