Skip Navigation



Hum. Reprod. Advance Access published online on August 7, 2006
Human Reproduction, doi:10.1093/humrep/del257
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
21/11/2960    most recent
del257v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Schweigert, F. J.
Right arrow Articles by Dudenhausen, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schweigert, F. J.
Right arrow Articles by Dudenhausen, J. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received April 12, 2006
Revised May 12, 2006
Accepted May 18, 2006

Article

Peptide and protein profiles in serum and follicular fluid of women undergoing IVF

Florian J. Schweigert 1 *, Beate Gericke 1, Wiebke Wolfram 1, Udo Kaisers 2, and Joachim W. Dudenhausen 3

1 Institute of Nutritional Science, University of Potsdam, Potsdam-Rehbrücke, Germany
2 Department of Anesthesiology and Intensive Care Medicine, University Medicine Berlin, Berlin, Germany
3 Department of Obstetrics, University Medicine Berlin, Berlin, Germany

* To whom correspondence should be addressed.
Florian J. Schweigert, E-mail: schweigert{at}nutriproteomics.de


  Abstract

BACKGROUND: Proteins and peptides in human follicular fluid originate from plasma or are produced by follicular structures. Compositional changes reflect oocyte maturation and can be used as diagnostic markers. The aim of the study was to determine protein and peptide profiles in paired serum and follicular fluid samples from women undergoing IVF. METHODS: Surface-enhanced laser desorption and ionization-time of flight-mass spectrometry (SELDI-TOF-MS) was used to obtain characteristic protein pattern. RESULTS: One hundred and eighty-six individual MS signals were obtained from a combination of enrichment on strong anion exchanger (110), weak cation exchanger (52) and normal phase surfaces (24). On the basis of molecular masses, isoelectric points and immunoreactivety, four signals were identified as haptoglobin ({alpha}1- and {alpha}2-chain), haptoglobin 1 and transthyretin (TTR). Immunological and MS characteristics of the TTR : retinol-binding protein (RBP) transport complex revealed no microheterogeneity differences between serum and follicular fluid. Discriminatory patterns arising from decision-tree-based classification and regression analysis distinguished between serum and follicular fluid with a sensitivity and specificity of 100%. CONCLUSIONS: Quantitative and qualitative differences indicate selective transport processes rather than mere filtration across the blood-follicle barrier. Identified proteins as well as characteristic peptide and/or protein signatures might emerge as potential candidates for diagnostic markers of follicle and/or oocyte maturation and thus oocyte quality.

Keywords: human follicular fluid/peptide/protein/proteome/serum.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
G. Zhao, Z. Li, K. Araki, K. Haruna, K. Yamaguchi, M. Araki, M. Takeya, Y. Ando, and K.-i. Yamamura
Inconsistency between hepatic expression and serum concentration of transthyretin in mice humanized at the transthyretin locus
Genes Cells, December 1, 2008; 13(12): 1257 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. R. Shaikly, I. E. G. Morrison, M. Taranissi, C. V. Noble, A. D. Withey, R. J. Cherry, S. M. Blois, and N. Fernandez
Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression
J. Immunol., March 15, 2008; 180(6): 4330 - 4337.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.