Hum. Reprod. Advance Access first published online on July 18, 2006
This version published online on August 18, 2006
Human Reproduction, doi:10.1093/humrep/del261
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Gynaecology, Université Catholique de Louvain, Brussels, Belgium
* To whom correspondence should be addressed. BACKGROUND: Iron deposits are characteristic of endometriotic lesions, and pelvic iron concentrations are higher in endometriosis patients than in women without endometriosis. In this study, the effect of iron overload and iron chelation on the development of endometriosis in a murine model was investigated. METHODS: Human menstrual endometrium was injected i.p. into nude mice, either alone (controls) or supplemented with erythrocytes or desferrioxamine (DFO), an iron chelator. After 5 days, the iron load of endometriosis-like lesions and peritoneal macrophages and fluid was evaluated. Lesions were quantified by immunohistochemical morphometry, and their proliferative activity was assessed. RESULTS: Injection of erythrocytes into the pelvic cavity caused iron overload in lesions (P < 0.025) and peritoneal macrophages (P < 0.01) and fluid (P < 0.05), whereas DFO effectively reduced iron status in lesions (P < 0.05) and macrophages (P < 0.01) compared with controls. No difference was observed in the number or surface area of lesions between the three groups. Erythrocytes increased (P < 0.05) and DFO significantly decreased (P < 0.01) the proliferative activity of lesions. CONCLUSIONS: Iron overload does not appear to affect lesion establishment but may contribute to the further growth of endometriosis by promoting cell proliferation of lesions. Iron chelator treatment could therefore be beneficial in endometriosis to prevent iron overload in the pelvic cavity and decrease cellular proliferation of lesions. This article is now available as the press embargo it was under has been lifted. This is a new version of this article as an error in an author name has been corrected.
Received March 29, 2006
Revised May 29, 2006
Accepted June 5, 2006
Article
Iron overload enhances epithelial cell proliferation in endometriotic lesions induced in a murine model
Sylvie Defrère 1, Anne Van Langendonckt 1, Sophie Vaesen 1, Mathieu Jouret 1, Reinaldo González Ramos 1, Dolores Gonzalez 1, and Jacques Donnez 1 *
Jacques Donnez, E-mail: donnez{at}gyne.ucl.ac.be
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Defrere, J.C. Lousse, R. Gonzalez-Ramos, S. Colette, J. Donnez, and A. Van Langendonckt Potential involvement of iron in the pathogenesis of peritoneal endometriosis Mol. Hum. Reprod., July 1, 2008; 14(7): 377 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Dassen, R. Kamps, C. Punyadeera, F. Dijcks, A. de Goeij, A. Ederveen, G. Dunselman, and P. Groothuis Haemoglobin expression in human endometrium Hum. Reprod., March 1, 2008; 23(3): 635 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gonzalez-Ramos, J. Donnez, S. Defrere, I. Leclercq, J. Squifflet, J.-C. Lousse, and A. Van Langendonckt Nuclear factor-kappa B is constitutively activated in peritoneal endometriosis Mol. Hum. Reprod., July 1, 2007; 13(7): 503 - 509. [Abstract] [Full Text] [PDF]
|
|