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Hum. Reprod. Advance Access published online on August 7, 2006
Human Reproduction, doi:10.1093/humrep/del301
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received March 6, 2006
Revised June 28, 2006
Accepted July 3, 2006

Article

Impact of gonadotrophins and steroid hormones on tumour cells derived from borderline ovarian tumours

Claire Basille 1, François Olivennes 2, Josyane Le Calvez 3, Nadine Beron-Gaillard 3, Geri Meduri 4, Catherine Lhommé 5, Pierre Duvillard 6, Jean Benard 3, and Philippe Morice 1 *

1 Departement de Chirurgie, Institut Gustave Roussy, Villejuif, France
2 Hopital Cochin, Paris, France
3 Departement de Biologie et de Pathologie Médicale, Institut Gustave Roussy, Villejuif, France
4 INSERM Unité 693, Le Kremlin-Bicêtre, France
5 Departement de Medecine, Institut Gustave Roussy, Villejuif, France
6 Departement de Pathologie, Institut Gustave Roussy, Villejuif, France

* To whom correspondence should be addressed.
Philippe Morice, E-mail: morice{at}igr.fr


  Abstract

BACKGROUND: Conservative surgery is currently proposed for young patients with borderline ovarian tumours (BOT). For those experiencing infertility, the question of medically assisted procreation is raised. We have evaluated in vitro the proliferation of cultured BOT cells in response to FSH or estradiol (E2). METHODS: Primary cell cultures were prepared from BOT. The presence of FSH and E2 receptors was evaluated by immunochemistry. Cultures in vitro were stimulated with FSH (40 and 200 mUI/ml) or E2 (300 and 2000 pg/ml) for 96 h and proliferation was evaluated with the WST-1 test. RESULTS: Four primary cultures were obtained that expressed FSH and E2 receptors to different extents. Growth was generally similar to controls when treated with either FSH or E2 although 300 pg/ml E2 caused a significant inhibitory effect on cell proliferation (P = 0.035). CONCLUSION: No stimulatory effect of FSH or E2 on cultured BOT cells was found, despite the presence of receptors. Although preliminary, these results suggest that gonadotrophins and E2 could be used in patients experiencing infertility after conservative surgery.

Keywords: borderline ovarian tumours/estradiol/FSH/gonadotrophin/infertility.
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