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Hum. Reprod. Advance Access published online on October 4, 2006
Human Reproduction, doi:10.1093/humrep/del312
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received April 18, 2006
Revised June 29, 2006
Accepted July 6, 2006

Article

Intrauterine administration of autologous peripheral blood mononuclear cells promotes implantation rates in patients with repeated failure of IVF-embryo transfer

S. Yoshioka 1, H. Fujiwara 1 *, T. Nakayama 1, K. Kosaka 1, T. Mori 2, and S. Fujii 1

1 Department of Gynecology and Obstetrics, Kyoto University, Sakyo-ku, Kyoto, Japan
2 Infertility Center, Daigo-Watanabe Clinic, Fushimi-ku, Kyoto, Japan

* To whom correspondence should be addressed.
H. Fujiwara, E-mail: fuji{at}kuhp.kyoto-u.ac.jp


  Abstract

BACKGROUND: There are few effective approaches to infertile patients with repeated failure in IVF-embryo transfer therapy. Since recent evidence suggests that some populations of maternal immune cells positively support embryo implantation, we have developed a new approach using peripheral blood mononuclear cells (PBMCs). METHODS: Patients who had not experienced successful pregnancy despite four or more IVF-embryo transfer sessions were enrolled in this study (n = 35, 35 cycles). PBMCs were obtained from patients on the day of oocyte retrieval and were cultured with HCG for 48 h. Two days later, PBMCs were freshly isolated from patients again, combined with cultured PBMC and then administered to the intrauterine cavity of the patients. Blastocyst transfer was performed on day 5, and the success of implantation in the PBMC-treated group was compared with that in the non-treated group. RESULTS: Clinical pregnancy rate, implantation rate and live birth rate in the PBMC-treated group (41.2, 23.4 and 35.3%; n = 17, 47 and 16, respectively) were significantly higher than those in the non-treated group (11.1, 4.1 and 5.5%; n = 18, 49 and 18, respectively). CONCLUSION: Intrauterine administration of autologous PBMC may be an effective approach to improve embryo implantation in patients with repeated IVF failures.

Keywords: embryo implantation/PBMC/IVF outcome.
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