Skip Navigation



Hum. Reprod. Advance Access published online on August 12, 2006
Human Reproduction, doi:10.1093/humrep/del328
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
22/1/36    most recent
del328v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Battersby, S.
Right arrow Articles by Jabbour, H.N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Battersby, S.
Right arrow Articles by Jabbour, H.N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received May 19, 2006
Revised July 10, 2006
Accepted July 17, 2006

Article

Seminal plasma and prostaglandin E2 up-regulate fibroblast growth factor 2 expression in endometrial adenocarcinoma cells via E-series prostanoid-2 receptor-mediated transactivation of the epidermal growth factor receptor and extracellular signal-regulated kinase pathway

S. Battersby 1, K.J. Sales 1, A.R. Williams 2, R.A. Anderson 3, S. Gardner 1, and H.N. Jabbour 1 *

1 Medical Research Council Human Reproductive Science Unit, Centre for Reproductive Biology, The Queen’s Medical Research Institute, Little France Crescent, Edinburgh, UK
2 Department of Pathology, Centre for Reproductive Biology, The Queen’s Medical Research Institute, Little France Crescent, Edinburgh, UK
3 Reproductive and Developmental Sciences, Centre for Reproductive Biology, The Queen’s Medical Research Institute, Little France Crescent, Edinburgh, UK

* To whom correspondence should be addressed.
H.N. Jabbour, E-mail: h.jabbour{at}hrsu.mrc.ac.uk


  Abstract

BACKGROUND: Prostaglandin E2 (PGE2) has been shown to modulate angiogenesis and tumour progression via the E-series prostanoid-2 (EP2) receptor. Endometrial adenocarcinomas may be exposed to endogenous PGE2 and exogenous PGE2, present at high concentration in seminal plasma. METHODS: This study investigated fibroblast growth factor 2 (FGF2) mRNA expression and cell signalling in response to seminal plasma or PGE2, using an endometrial adenocarcinoma (Ishikawa) cell line stably expressing the EP2 receptor (EP2 sense cells) and endometrial adenocarcinoma explants. RESULTS: Seminal plasma and PGE2 induced a significant up-regulation of FGF2 expression in EP2 sense but not parental untransfected Ishikawa (wild-type) cells (P < 0.05). These effects were inhibited by cotreatment with EP2 receptor antagonist or inhibitors of protein kinase A, c-Src, epidermal growth factor receptor (EGFR) kinase or extracellular signal-regulated kinase (ERK) signalling. The treatment of EP2 sense cells with seminal plasma induced cAMP accumulation and phosphorylation of c-Src, EGFR kinase and ERK via the EP2 receptor. Finally, seminal plasma and PGE2 significantly increased FGF2 mRNA expression in endometrial adenocarcinoma tissue explants via the EP2 receptor (P < 0.05). CONCLUSIONS: Seminal plasma and PGE2 can similarly activate FGF2 expression and EP2 receptor signalling in endometrial adenocarcinoma cells. These data highlight the potential for seminal plasma exposure to facilitate tumorigenesis-angiogenesis in endometrial adenocarcinomas in vivo.

Keywords: endometrial carcinoma/E-series prostanoid receptor 2/fibroblast growth factor 2/seminal plasma.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
Y. Zheng, J. D. Ritzenthaler, X. Sun, J. Roman, and S. Han
Prostaglandin E2 Stimulates Human Lung Carcinoma Cell Growth through Induction of Integrin-Linked Kinase: The Involvement of EP4 and Sp1
Cancer Res., February 1, 2009; 69(3): 896 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Jain, G. Chakraborty, R. Raja, S. Kale, and G. C. Kundu
Prostaglandin E2 Regulates Tumor Angiogenesis in Prostate Cancer
Cancer Res., October 1, 2008; 68(19): 7750 - 7759.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. J. Sales, S. C. Boddy, A. R. W. Williams, R. A. Anderson, and H. N. Jabbour
F-Prostanoid Receptor Regulation of Fibroblast Growth Factor 2 Signaling in Endometrial Adenocarcinoma Cells
Endocrinology, August 1, 2007; 148(8): 3635 - 3644.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.