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Hum. Reprod. Advance Access published online on August 18, 2006
Human Reproduction, doi:10.1093/humrep/del330
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received April 20, 2006
Revised May 19, 2006
Accepted July 17, 2006

Article

The asynaptic chromatin in spermatocytes of translocation carriers contains the histone variant {gamma}-H2AX and associates with the XY body

R. Sciurano 1, M. Rahn 1, G. Rey-Valzacchi 2, and A.J. Solari 1 *

1 Biologia Celular, CIR, Facultad de Medicina, Universidad de Buenos Aires
2 PROCREARTE, Argentina

* To whom correspondence should be addressed.
A.J. Solari, E-mail: asolari{at}fmed.uba.ar


  Abstract

BACKGROUND: The close apposition of multivalents with the XY body has been repeatedly described in heterozygous carriers of chromosomal rearrangements. Because in many of these carriers spermatogenesis is deeply disturbed at the spermatocyte level, the association of autosomal chromatin with the XY body may impair the spermatocyte life. METHODS: Testicular biopsies from three men carriers of three different chromosomal rearrangements have been analysed by electron microscopy (EM) and immunolocalization of meiotic proteins. RESULTS: There is an ordered transition from isolated multivalents at early pachytene to XY body association in late pachytene, as shown in a carrier of a rob t(13;14) translocation by EM and in a reciprocal translocation t(9;14) carrier by immunofluorescence. The non-synapsed ends of the quadrivalent show BRCA1 located on the axes and the variant histone {gamma}-H2AX located on the chromatin. The area covered by {gamma}-H2AX increases with the association of the asynaptic ends with the XY body in the t(9;14) carrier, and the area covered with {gamma}-H2AX in the t(Y;15) carrier is larger than that of the XY body of controls. CONCLUSIONS: The affinity between the inactive XY body and asynaptic regions of multivalents is given a material basis, and transcriptional inactivation is probably shared by these two chromatin types.

Keywords: chromosome rearrangements/meiosis/spermatogenesis impairment/XY body/{gamma}-H2AX.
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