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Hum. Reprod. Advance Access published online on August 21, 2006
Human Reproduction, doi:10.1093/humrep/del332
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received April 12, 2006
Revised July 7, 2006
Accepted July 17, 2006

Article

Identification, characterization and co-localization of label-retaining cell population in mouse endometrium with typical undifferentiated markers

I. Cervelló 1, J.A. Martínez-Conejero 1, J.A. Horcajadas 1, A. Pellicer 1, and C. Simón 2 *

1 Fundación Instituto Valenciano de Infertilidad (FIVI)--Instituto Universitario IVI--Valencia University, Valencia, Spain
2 Fundación Instituto Valenciano de Infertilidad (FIVI)--Instituto Universitario IVI--Valencia University, Valencia, Spain; Valencia Stem Cell Bank, Centro de Investigación Príncipe Felipe, Valencia, Spain

* To whom correspondence should be addressed.
C. Simón, E-mail: csimon{at}ivi.es


  Abstract

BACKGROUND: The endometrium, lining of the uterus, is a highly active organ that is remodelled periodically during the lifespan. Different studies suggest the presence of an adult or progenitor stem cell (PSC) population in this tissue because of its cyclic regenerative capacity. METHODS: In this study, we aim at identifying and localizing the putative PSC population in the murine uterus using the 5-bromo-2'deoxyuridine (BrdU) labelling method to detect label-retaining cells (LRCs) that cycle slowly. Uteri from BrdU-treated mice were analysed via single and double immunohistochemistry to co-localize them with the markers of undifferentiation already described such as c-KIT and POU5F1 (also known as OCT-4). Finally, we confirmed the presence of the indicated markers at mRNA level. RESULTS: We observed the presence and gradual decrease of LRCs in the endometrium during the lifespan of the mice. In adulthood, the LRC population decreased notably and remained in the lower region of the stroma in the murine endometrium. Some of the endometrial LRCs co-localized with c-KIT and POU5F1. PCR and nested-PCR confirmed the presence of these undifferentiated markers. CONCLUSIONS: We demonstrated that the murine endometrium possesses LRCs with the features of a putative PSC population localized at the lower region of the stroma.

Keywords: BrdU-retaining cells/endometrium/progenitor stem cells/undifferentiation.
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