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Hum. Reprod. Advance Access published online on September 11, 2006
Human Reproduction, doi:10.1093/humrep/del354
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received May 15, 2006
Revised July 31, 2006
Accepted August 9, 2006

Article

Successful delivery after the transfer of twice-vitrified embryos derived from in vitro matured oocytes: A Case Report

S. Hashimoto 1 *, Y. Murata 1, M. Kikkawa 1, M. Sonoda 1, H. Oku 1, T. Murata 2, K. Sugihara 2, F. Nagata 2, Y. Nakaoka 2, A. Fukuda 2, and Y. Morimoto 1

1 IVF NAMBA CLINIC, The Centre for Reproductive Medicine and Infertility, Osaka, Japan
2 IVF OSAKA CLINIC, Osaka, Japan

* To whom correspondence should be addressed.
S. Hashimoto, E-mail: hashimoto{at}ivfnamba.com


  Abstract

We report here the first case of successful pregnancy and delivery after the blastocyst transfer of twice-vitrified embryos produced following in vitro maturation (IVM) and ICSI. The patient received 5000 IU hCG on day 12 of the treatment cycle, and oocyte retrieval was carried out 36 h after hCG injection. A total of 22 immature oocytes were obtained. Following incubation for 26 h in IVM medium, 15 oocytes (68.2%) reached metaphase II stage. In total, 13 oocytes (86.7%) were fertilized after ICSI with the husband’s sperm, and 11 embryos at the pronuclear stage and two cleaved embryos on day 2 were vitrified because of thin endometrial thickness. Eight cryopreserved embryos at the pronuclear stage were warmed and cultured until the day 3 stage. Three embryos were transferred, and three embryos were twice vitrified. Unfortunately, these transferred embryos did not implant. Three twice-vitrified embryos were rewarmed and cultured until the day 5 stage, and two embryos were transferred. The second transfer attempt of twice-vitrified embryos resulted in the full-term delivery of a healthy infant. This case report demonstrates that twice-vitrified embryos, developed using an IVM protocol, retain the developmental competence for full-term, healthy infants.

Keywords: in vitro maturation/PCOS/repeated cryopreservation/vitrification.
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