Skip Navigation



Hum. Reprod. Advance Access published online on September 22, 2006
Human Reproduction, doi:10.1093/humrep/del357
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
22/1/201    most recent
del357v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Strassburger, D.
Right arrow Articles by Raziel, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strassburger, D.
Right arrow Articles by Raziel, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received September 1, 2005
Revised August 5, 2006
Accepted August 11, 2006

Article

Morphology assessment and fluorescence in situ hybridization of the same spermatozoon using a computerized cell-scanning system

D. Strassburger 1 *, M. Reichart 2, S. Kaufman 1, E. Kasterstein 1, D. Komarovsky 1, O. Bern 1, S. Friedler 1, M. Schachter 1, R. Ron-El 1, and A. Raziel 1

1 Infertility and IVF Unit Assaf Harofeh Medical Center, Tel-Aviv University, Zerifin, Israel
2 BioView Ltd, Nes Ziona, Israel

* To whom correspondence should be addressed.
D. Strassburger, E-mail: dvoras{at}asaf.health.gov.il


  Abstract

BACKGROUND: Poor sperm morphology is statistically associated with an increase in the incidence of chromosome abnormalities. Our aim was to examine the possible correlation between chromosomal aberrations and sperm morphology in the same cell. METHODS: 12349 spermatozoa from 7 teratozoospermic and one globozoospermic patients, and from 3 fertile donors were analyzed using a system which scans for cell morphology and chromosomal ploidy in the same cell using digital technology. RESULTS: Chromosomal aberrations were detected in 5.3% of teratozoospermic cases and in 6.7% in the globozoospermic patient compared with 1.6% in donors (P < 0.0001). Chromosomal aberrations were more common in abnormally formed sperm compared with normal spermatozoa: 4.5% vs 1.3% in the teratozoospermic group and 2.0% vs 0.3% in the control group (NS), especially frequent among sperm with two heads or two tails (52.1-77.2%) or extreme head deformations (10.6-11.1%) irrespective of grouping, and in mild amorphous heads in the globozoospermic patients (20.2%). The frequency of chromosomal aberrations in morphologically normal sperm was comparable whether derived from teratozoospermic or normospermic patients. CONCLUSIONS: The computerized cell-scanning system demonstrated the relationship between chromosomal aberrations and sperm morphology in the same spermatozoon. The incidence of chromosomal aberrations was positively linked to abnormal sperm morphology, the more severe the abnormality, the higher the incidence of aneuploidy.

Keywords: aneuploidy/fluorescence in situ hybridization/globozoospermia/morphological abnormalities/teratozoospermia.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
G. Cavallini, A. Crippa, M. C. Magli, N. Cavallini, A. P. Ferraretti, and L. Gianaroli
A Study to Sustain the Hypothesis of the Multiple Genesis of Oligoasthenoteratospermia in Human Idiopathic Infertile Males
Biol Reprod, October 1, 2008; 79(4): 667 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
N. Prisant, D. Escalier, J-C. Soufir, M. Morillon, D. Schoevaert, M. Misrahi, and G. Tachdjian
Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads
Hum. Reprod., April 1, 2007; 22(4): 1052 - 1059.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.