Skip Navigation



Hum. Reprod. Advance Access published online on November 2, 2006
Human Reproduction, doi:10.1093/humrep/del418
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
22/3/733    most recent
del418v2
del418v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Geens, M.
Right arrow Articles by Tournaye, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geens, M.
Right arrow Articles by Tournaye, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received June 7, 2006
Revised August 30, 2006
Accepted September 21, 2006

Article

The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients

M. Geens 1 *, H. Van de Velde 2, G. De Block 1, E. Goossens 1, A. Van Steirteghem 2, and H. Tournaye 2

1 Research Centre for Reproduction and Genetics, University Hospital and Medical School, Vrije Universiteit Brussel, Laarbeeklaan 101, B-1090 Brussels, Belgium
2 Centre for Reproductive Medicine, University Hospital and Medical School, Vrije Universiteit Brussel, Brussels, Belgium

* To whom correspondence should be addressed.
M. Geens, E-mail: mieke.geens{at}az.vub.ac.be


  Abstract

BACKGROUND: Before clinical application, the feasibility and safety of autologous testicular stem cell transplantation should be explored. Apart from limitations in their numbers, spermatogonial stem cells may also be contaminated by malignant cells. Therefore, both enrichment and decontamination before transplantation may be necessary. This study aimed at evaluating the decontaminating potential of magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) for both murine and human testicular cell suspensions. In the mouse, the effectiveness of the transplantation technique after cell sorting was also assessed. METHODS: Murine testicular cells were contaminated with 5% EL4 cells. Fresh and frozen-thawed suspensions were sorted using MACS (CD49f+) and FACS (CD49f+, H-2Kb-) and evaluated by FACS, cell culture and transplantation into W/Wv mice. Human testicular cells were contaminated with 5 or 0.05% CCRF-SB (SB) cells. Frozen-thawed suspensions were sorted using FACS (HLA class I-) and evaluated by FACS, cell culture and PCR for the B-cell receptor. RESULTS: In the mouse, the sorted fractions contained 0.39% H-2Kb-positive and 76.55% CD49f-positive cells. After transplantation, 1 in 20 recipient mice developed a malignancy. In the human experiments, an average of 0.58% SB cells was detected after sorting. In only 1 of 11 samples, there were no SB cells observed. CONCLUSION: MACS and/or FACS are insufficient for completely depleting testicular tissue of malignant cells. Although more research on alternative decontamination techniques is necessary, developing a reliable method to screen a priori testicular tissue for malignant cells may be equally important.

Keywords: spermatogonia/decontamination/transplantation/cancer.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JAMAHome page
H. Sadri-Ardekani, S. C. Mizrak, S. K. M. van Daalen, C. M. Korver, H. L. Roepers-Gajadien, M. Koruji, S. Hovingh, T. M. de Reijke, J. J.M.C.H. de la Rosette, F. van der Veen, et al.
Propagation of Human Spermatogonial Stem Cells In Vitro
JAMA, November 18, 2009; 302(19): 2127 - 2134.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Goossens, M. De Rycke, P. Haentjens, and H. Tournaye
DNA methylation patterns of spermatozoa and two generations of offspring obtained after murine spermatogonial stem cell transplantation
Hum. Reprod., September 1, 2009; 24(9): 2255 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Marc Luetjens, J.-B. Stukenborg, E. Nieschlag, M. Simoni, and J. Wistuba
Complete Spermatogenesis in Orthotopic But Not in Ectopic Transplants of Autologously Grafted Marmoset Testicular Tissue
Endocrinology, April 1, 2008; 149(4): 1736 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
M. Geens, E. Goossens, G. De Block, L. Ning, D. Van Saen, and H. Tournaye
Autologous spermatogonial stem cell transplantation in man: current obstacles for a future clinical application
Hum. Reprod. Update, March 1, 2008; 14(2): 121 - 130.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
T. M. Said, A. Agarwal, M. Zborowski, S. Grunewald, H.-J. Glander, and U. Paasch
Utility of Magnetic Cell Separation as a Molecular Sperm Preparation Technique
J Androl, March 1, 2008; 29(2): 134 - 142.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. Hou, M. Andersson, C. Zheng, A. Sundblad, O. Soder, and K. Jahnukainen
Decontamination of leukemic cells and enrichment of germ cells from testicular samples from rats with Roser's T-cell leukemia by flow cytometric sorting
Reproduction, December 1, 2007; 134(6): 767 - 779.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Geens, H. Van de Velde, G. De Block, E. Goossens, and H. Tournaye
Reply: Isolation of germ cells from leukaemic cells
Hum. Reprod., October 1, 2007; 22(10): 2797 - 2798.
[Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Fujita, A. Tsujimura, and A. Okuyama
Isolation of germ cells from leukemic cells
Hum. Reprod., October 1, 2007; 22(10): 2796 - 2797.
[Full Text] [PDF]

Home page
 Reproductive SciencesHome page
C. E. Gargett
Review Article: Stem Cells in Human Reproduction
Reproductive Sciences, July 1, 2007; 14(5): 405 - 424.
[Abstract] [PDF]

Home page
 Hum ReprodHome page
M. Hou, M. Andersson, S. Eksborg, O. Soder, and K. Jahnukainen
Xenotransplantation of testicular tissue into nude mice can be used for detecting leukemic cell contamination
Hum. Reprod., July 1, 2007; 22(7): 1899 - 1906.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.