Hum. Reprod. Advance Access published online on November 1, 2006
Human Reproduction, doi:10.1093/humrep/del427
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1 Institut Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clinic, C/Casanova 143, 08036 Barcelona, Spain
* To whom correspondence should be addressed. BACKGROUND: Vascular endothelial growth factor (VEGF) has been investigated as a marker of ovarian response to controlled ovarian hyperstimulation and as a predictor of ovarian hyperstimulation syndrome (OHSS) in IVF cycles. In most studies, serum has been used for circulating VEGF concentration measurement, but it has been suggested that plasma is the preferred medium to measure VEGF levels because of the potential contribution of VEGF released from platelets during blood clotting. This study investigated VEGF concentrations in paired serum and plasma samples from patients undergoing controlled ovarian hyperstimulation for IVF. METHODS: Serum and plasma VEGF levels, as well as the number of platelets, were measured in 30 IVF patients who comprised three study groups delineated according to the estradiol (E2) serum concentration reached on the day of HCG administration:10 patients having low E2 serum levels (<1500 pg/ml, group L), 10 patients having intermediate E2 serum levels (1500-3000 pg/ml, group I) and 10 patients having high E2 serum levels (>3000 pg/ml, group H). RESULTS: There was a statistically significant correlation between plasma and serum VEGF levels (
Received July 19, 2006
Revised September 29, 2006
Accepted October 5, 2006
Article
Vascular endothelial growth factor levels in serum and plasma from patients undergoing controlled ovarian hyperstimulation for IVF
Dolors Manau 1, Francisco Fábregues 1, Joana Peñarrubia 1, Montserrat Creus 1, Francisco Carmona 1, Gemma Casals 1, Wladimiro Jiménez 2, and Juan Balasch 1 *
2 Department of Biochemistry and Molecular Genetics, Faculty of Medicine--University of Barcelona, Hospital Clinic-Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
Juan Balasch, E-mail: jbalasch{at}ub.edu
Abstract 
= 0.61; P < 0.005) for the entire population studied, although serum values were higher by a factor of 
6-fold. No significant correlation was found between peripheral blood VEGF concentrations and serum E2 or follicle number on HCG day or the number of oocytes collected. Similarly, paired serum and plasma VEGF measurements did not correlate with platelet count. CONCLUSIONS: Serum and plasma VEGF concentrations are strongly correlated in paired samples from infertile patients undergoing controlled ovarian hyperstimulation. However, neither serum nor plasma VEGF levels were correlated with parameters associated with ovarian follicular activity. Peripheral blood VEGF levels were not correlated with platelet count.
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