Skip Navigation



Hum. Reprod. Advance Access published online on April 20, 2007
Human Reproduction, doi:10.1093/humrep/dem070
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
22/6/1681    most recent
dem070v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zeng, H.-t.
Right arrow Articles by Liang, X.-y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeng, H.-t.
Right arrow Articles by Liang, X.-y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Low mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytes

Hai-tao Zeng1, Zi Ren1, William S.B. Yeung2, Yi-min Shu1,3, Yan-wen Xu1, Guang-lun Zhuang1 and Xiao-yan Liang1,4

1 Centre for reproductive medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, P.R. China 2 Department of Obstetrics and Gynecology, University of Hong Kong, Hong Kong, Special Administrative Region, P.R. China 3 IVF Program, Stanford University Medical Center, Palo Alto, CA 94304, USA

4 Correspondence address. Centre for reproductive medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, P.R. China. E-mail: lxyzy{at}yahoo.com

BACKGROUND: Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated the relationship between birefrigent meiotic spindle and cytoplasmic mitochondrial DNA (mtDNA) and ATP contents in in vitro matured human oocytes.

METHODS: Oocytes at germinal vesicle stage were collected and cultured for 24–48 h with or without the metabolic inhibitor, carbonyl cyanide p-(tri-fluromethoxy) phenyl-hydrazone (FCCP). All in vitro matured oocytes were examined by PolScope for the presence of meiotic spindle, then the oocytes were used for either intracytoplasmic sperm injection or the measurement of mitochondrial quantity and ATP content.

RESULTS: Meiotic spindles were observed in 51.3% (60/117) of the in vitro matured oocytes. Oocytes with detectable meiotic spindle contained significantly higher mtDNA copies (637 250 ± 237 606 versus 491 454 ± 153 406, P = 0.027) and ATP content (1.97 ± 0.38 versus 1.65 ± 0.32 pmol, P = 0.028) when compared with those without detectable meiotic spindle. However, in vitro matured oocytes showed a significantly reduced rate of positive meiotic spindle and a lower ATP content when cultured with FCCP. A lower incidence of normal fertilization and good quality embryos were observed if meiotic spindles were not detected.

CONCLUSIONS: Low mtDNA and ATP content might contribute to the absence of birefringent spindle imaged with the PolScope in human in vitro matured oocytes.

Key words: mitochondria/spindle/oocyte/PolScope/embryo development

Submitted on February 8, 2007; resubmitted on February 19, 2007; accepted on February 21, 2007.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
J. Van Blerkom, P. Davis, and V. Thalhammer
Regulation of mitochondrial polarity in mouse and human oocytes: the influence of cumulus derived nitric oxide
Mol. Hum. Reprod., August 1, 2008; 14(8): 431 - 444.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. Van Blerkom and P. Davis
Mitochondrial signaling and fertilization
Mol. Hum. Reprod., November 1, 2007; 13(11): 759 - 770.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.