Skip Navigation



Hum. Reprod. Advance Access published online on May 2, 2008
Human Reproduction, doi:10.1093/humrep/den130
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
23/7/1485    most recent
den130v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dalgetty, D.M.
Right arrow Articles by Horne, A.W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dalgetty, D.M.
Right arrow Articles by Horne, A.W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Altered secretory leukocyte protease inhibitor expression in the uterine decidua of tubal compared with intrauterine pregnancy

D.M. Dalgetty1, J.M. Sallenave2,3, H.O.D. Critchley4, A.R. Williams5, W.Y. Tham4, A.E. King4 and A.W. Horne4,6

1 MRC Human Reproductive Sciences Unit, The Queen’s Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK 2 Université Denis Diderot-Paris 7, France 3 Unité de Défense Innée et Inflammation, INSERM U874, Batiment Metchnikoff, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris Cedex, France 4 Department of Reproductive and Developmental Sciences, The University of Edinburgh, Centre for Reproductive Biology, The Queen’s Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK 5 Department of Pathology, The University of Edinburgh, Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, UK

6 Correspondence address. E-mail: andrew.horne{at}ed.ac.uk

BACKGROUND: The role of the innate immune system in tubal implantation remains undefined. This study compared expression of two key mediators of innate immunity, secretory leukocyte protease inhibitor (SLPI) and elafin, in the uterine decidua of women with intrauterine and tubal pregnancies.

METHODS: Uterine decidua was collected from women (18–45 years) undergoing surgical termination of pregnancy (n = 7), surgical management of spontaneous abortion (n = 6) and tubal pregnancy (n = 10). Using quantitative RT–PCR and immunohistochemistry, mRNA and protein expression patterns of SLPI and elafin were compared.

RESULTS: Relative SLPI mRNA expression was significantly higher in decidua of women with tubal pregnancy (12.37 ± 2.66) compared with spontaneous abortion (5.09 ± 2.22, P < 0.0185). There was no difference demonstrated in elafin mRNA expression. SLPI and elafin protein expression were demonstrated in the decidual leukocyte populations and epithelium. There was no obvious qualitative difference in levels of SLPI and elafin protein expression or their distribution in the uterine decidua of women with termination of pregnancy, spontaneous abortion or tubal pregnancy.

CONCLUSIONS: Herein we demonstrate novel differences in gene expression of uterine decidua of tubal pregnancy compared with spontaneous abortion thereby contributing further to current knowledge of mechanisms involved in extrauterine implantation. The altered expression of SLPI may be a consequence of, or predispose to, tubal pregnancy.

Key words: secretory leukocyte protease inhibitor/elafin/uterine decidua/tubal pregnancy

Submitted on October 1, 2007; resubmitted on March 5, 2008; accepted on March 23, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
A.W. Horne, W.C. Duncan, A.E. King, S. Burgess, P.C. Lourenco, P. Cornes, P. Ghazal, A.R. Williams, L. Udby, and H.O.D. Critchley
Endometrial cysteine-rich secretory protein 3 is inhibited by human chorionic gonadotrophin, and is increased in the decidua of tubal ectopic pregnancy
Mol. Hum. Reprod., May 1, 2009; 15(5): 287 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. E. King, N. Wheelhouse, S. Cameron, S. E. McDonald, K.-F. Lee, G. Entrican, H. O.D. Critchley, and A. W. Horne
Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection
Hum. Reprod., March 1, 2009; 24(3): 679 - 686.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.