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Hum. Reprod. Advance Access published online on February 25, 2009
Human Reproduction, doi:10.1093/humrep/dep037
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© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Prognostic value of sperm fluorescence in situ hybridization analysis over PGD

M. Sánchez-Castro1, A.R. Jiménez-Macedo1, M. Sandalinas1 and J. Blanco2,3

1 Reprogenetics Spain, S.A., 08006 Barcelona, Spain 2 Unitat de Biologia Cel·lular, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain

3 Correspondence address. E-mail: Joan.Blanco{at}uab.cat

BACKGROUND: Sperm aneuploidy screening is now widely used as a counseling tool before performing a PGD cycle in infertile patients. The goal of this study was to determine whether sperm aneuploidy has a direct influence on embryo chromosomal abnormalities.

METHODS: Two groups were set up: (i) study group consisting of 13 oocyte-donation PGD cycles from males with normal karyotype and a previous altered sperm fluorescence in situ hybridization (FISH) result and (ii) control group including nine cycles of PGD for X-linked disease with no fertility problems and maternal age <36 years. Sperm samples and Day 3 embryos were evaluated using FISH for chromosomes X, Y, 13, 18 and 21.

RESULTS: A total of 179 embryos were analyzed: 91 embryos for the control group versus 88 for the study group. The study group presented more abnormal embryos than the control group (51.14% versus 35.16%; P = 0.0353). Patients from the study group were then classified according to sperm count. Oligozoospermic patients showed a much higher proportion of abnormal embryos compared with the control group (64.87% versus 35.16%; P = 0.0030).

CONCLUSIONS: Sperm aneuploidy and diploidy screening seems to be an effective prognostic tool that would be useful in the reproductive genetic counseling of infertile couples, especially in oligozoospermic patients.

Key words: sperm fluorescence in situ hybridization/PGD/oligozoospermia/genetic counseling/infertility

Submitted on November 13, 2008; resubmitted on January 22, 2009; accepted on January 26, 2009.


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